Обзор продукта
ПЦР amplification of DNA fragments, DNA labeling, primer extension, sequence determination, blunt end addition of A, TA vector cloning
| Информация о товаре | |
|---|---|
| Молекулярная масса | 94 КД |
| Polymerase Activity | 5′-3′ ДНК-полимераза, 5′-3′ exonuclease (no 3′-5′ exonuclease) |
| PCR Buffer | 10 X PCR Buffer for direct loading |
| Experimental Efficiency | The PCR reaction can be directly detected by electrophoresis |
| Extension Speed | 1-2 kb/min |
| Terminal Base | Product has A at the 3′ конец, facilitating TA vector cloning |
| Reaction System (20мкл) | Addition Amount | Примечания |
|---|---|---|
| Pure DNA Polymerase | 0.8мкл | Adjust for amplification needs |
| 10x PCR Buffer | 2мкл | |
| dNTP (2.5мм) | 2мкл | |
| PrimerF | 0.1-1мкл | 10-50pmol used |
| PrimerR | 0.1-1мкл | 10-50pmol used |
| Template | <1мкг | |
| ddH2O | Up to 20μl |
| Cycle Program | Температура (°С) | Время | Примечания |
|---|---|---|---|
| Initial Denaturation | 94 | 3мин | |
| Денатурация | 94 | 30с (30-40 циклы) | |
| Отжиг | 45-60 | 30с | |
| Расширение | 72 | 30s-2min | Extension time varies with fragment |
| Final Extension | 72 | 10мин |
Процедура
Can be directly detected without adding a Loading Buffer
Result Detection
После ПЦР, брать 5 μl of product for agarose gel electrophoresis
Отзывы
Отзывов пока нет.