Набор для ПЦР ДНК-полимеразы SENO Taq 200T

Доставка в долларах США 45 - Бесплатно свыше долларов США 300

DTE — китайская платформа электронной коммерции, специализирующаяся на онлайн-продажах молекулярных тестов., ИФА, и сопутствующие товары.

Спецификация

ДНК-полимераза Taq 1000 U with mixed buffer (Shipping with icebag)

Taq DNA Polymerase and Taq ПЦР Kits should be stored at -30 to -15°C in a constant-temperature freezer upon receipt.

Notes Before Starting:

Provided with Load PCR Buffer containing gel-loading reagent and gel-tracking dyes.
PCR Buffer and Load PCR Buffer yield a final concentration of 1.5 mM MgCl2 in the reaction mix. Adjust Mg2+ concentration if necessary by adding 25 mM MgCl2.
High-quality PCR-grade dNTP Mix (10 мм) is available separately if needed.
Keep PCR tubes on ice until placed in the thermal cycler.
Include a No Template Control (NTC) in every assay.

Thaw buffers and reagents at room temperature or on ice, then keep them on ice after complete thawing.
Prepare a reaction mix containing all PCR components except template DNA. Подготовить 10% more volume than required.
Mix the reaction mix thoroughly and dispense it into PCR tubes.
Add template DNA (≤1 µg/reaction) to PCR tubes. For RT-PCR, add an aliquot from the reverse transcriptase reaction, not exceeding 10% of the final PCR volume.

Компонент	Volume/reaction	Final concentration
Reaction mix
10x PCR Bufferˢ¹ or	10 мкл	1Икс
10x Load PCR Buffer(необязательный)
dNTP mix (10 mM of each)	2 мкл	200 µM of each dNTP
Primer1	Variable	0.1–0.5 µM
Primer2	Variable	0.1–0.5 µM
ДНК-полимераза Taq	0.5 мкл	2.5 units/reaction
RNAse-free water	Variable
Template DNA (added at step 4)	Variable	≤1 µg/reaction
Общий объем реакции	100 µlˢ²

Program thermal cycler according to manufacturer’s instructions. Refer to the provided cycling program.

Шаг	Время	Температура	Comment
Initial denaturation	3 мин	94°С
3-step cycling:
Денатурация	0.5–1 min	94°С
Отжиг	0.5–1 min	50–68°C	Approximately 5°C below Tm of primers.
Расширение	1 мин	72°С	For PCR products longer than 1 КБ, use an extension time of approximately 1 min per kb DNA.
Количество циклов	25–35
Final extension	10 мин	72°С

Start PCR program. Once the thermal cycler reaches 94°C, place PCR tubes in the cycler for improved specificity.

After amplification, samples can be stored at 2–8°C overnight or at -20°C for longer storage.
PCR products can be directly loaded onto agarose gel using Load PCR Buffer without prior addition of loading buffer and gel-tracking dyes. Refer to the provided table for migration distance and gel tracking dyes.