HiPure Circulating DNA Mini Kit, designed to efficiently purify short, cell-free DNA (cfDNA) molecules from human biological fluids:

• Target:
  • Tumor-derived cfDNA fragments and mRNAs in blood
  • Fetal nucleic acids in maternal blood
• Sample Compatibility:
  • Human plasma
  • Human serum
  • Human urine
  • Samples can be fresh or frozen (once only)
• Focus on Short DNA: The kit isolates short nucleic acids (<1000bp) commonly found in biofluids.
• Efficient Purification: The protocol allows for effective recovery of cfDNA from various biofluids.
• Ready for Downstream Use: Purified cfDNA is suitable for diverse applications, รวมทั้ง:
  • พีซีอาร์ and other amplification reactions
  • Next-generation sequencing (NGS) analyses

Benefits:

• Optimized for Short cfDNA: Specifically designed to capture short DNA fragments relevant for biomarker discovery and liquid biopsies.
• ความเข้ากันได้ของตัวอย่างในวงกว้าง: Works effectively with various human biofluids, offering flexibility in sample selection.
• Streamlined Workflow: The miniaturized kit format allows for faster processing compared to larger kits.

This kit empowers researchers to study circulating DNA in various fields, including oncology, non-invasive prenatal testing (NIPT), and liquid biopsy applications.

ข้อมูลจำเพาะ

หลักการ

This product utilizes silica gel columns for a rapid and reliable การทำให้ดีเอ็นเอบริสุทธิ์ process.

1. Lysis and Digestion: The sample undergoes treatment with a lysate solution and protease. This breaks open cells and degrades proteins, releasing DNA into the solution.

2. ยึดเกาะกับซิลิกาเมมเบรน: The lysate containing the DNA is transferred to an adsorption plate or filtration column. The silica membrane in these tools selectively binds DNA molecules due to favorable interactions, while proteins and other impurities pass through.

3. ซักผ้า: บัฟเฟอร์การซักจะถูกส่งผ่านคอลัมน์, removing unbound proteins and cellular debris that didn’t adhere to the silica.

4. ชะล้าง: A low-salt buffer (โดยทั่วไป 10 เอ็มเอ็ม ทริส, ค่า pH 8.0) ใช้เพื่อชะล้าง DNA บริสุทธิ์จากเยื่อหุ้มซิลิกา. This disrupts the interaction between DNA and the silica, allowing the collection of purified DNA in a separate tube.

This method provides a valuable tool for researchers working in various fields that require purified DNA for analysis, เช่น พีซีอาร์, การซับใต้, และการเรียงลำดับรุ่นต่อไป.

ข้อดี

• ให้ผลผลิตสูง – กระบวนการที่เหมาะสมที่สุด, free DNA (>50bp) can be obtained to the maximum extent
• High concentration – low elution volume, ensuring high nucleic acid concentration
• มีความบริสุทธิ์สูง – low alcohol binding method, completely removing inhibitor and protein pollution
• High recovery – DNA can be recovered at the level of PG by silica gel column purification

เนื้อหาชุด

การจัดเก็บและความเสถียร

Here’s a quick guide on storing your DNA extraction kit to ensure it functions at its best:

Ideal Storage:

• Store Proteinase K and Carrier RNA in the refrigerator (2-8องศาเซลเซียส) upon arrival for maximum effectiveness.

Acceptable Short-Term Storage:

• Proteinase K and Carrier RNA can be kept at room temperature (15-25องศาเซลเซียส) for up to 12 weeks without impacting their performance.

Storing Remaining Components:

• All other kit components should be stored dry at room temperature (15-25องศาเซลเซียส).
• They will remain stable for at least 18 เดือนภายใต้เงื่อนไขเหล่านี้.

Important Notes:

• If storing the entire kit at 2-8°C, remember to redissolve buffers before use.
• Always ensure all buffers reach room temperature before using them for optimal results.

By following these storage recommendations, you can guarantee your DNA extraction kit is always ready for use and delivers the best possible results.