การแนะนำ

The Kit provides fast purification of high-quality DNA, RNA and Prote from cells, เนื้อเยื่อ, and yeast using silica-membrane spin columns with a binding capacity of 100ug RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation, or precipitation with isopropanol or LiCl are eliminated. RNA purified using the HiPure Total RNA Purification System is ready for applications such as RT-พีซีอาร์, การซับภาคเหนือ, poly A+ RNA (เอ็มอาร์เอ็นเอ) purification, nuclease protection, and in vitro translation.

รายละเอียด

ข้อมูลจำเพาะ

หลักการ

The Kit isolates total RNA from up to 10 ⁷ cells or 30 mg tissue. A short workflow enables RNAisolation with genomic DNA removal in less than 25 นาที. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column, ethanol is added to the flow-through, and the sample is applied to an RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 30 µl water using the Kit.

ข้อดี

• High quality – high purity total RNA / DNA can be directly used in a variety of downstream applications
• เร็ว – column method can complete the extraction of several samples in 30 นาที
• ปลอดภัย – no phenol chloroform extraction required
• Simultaneous extraction- simultaneously isolate DNA and RNA from one sample

เนื้อหาชุด

การจัดเก็บและความเสถียร

HiPure Kit can be stored dry at room temperature (15–25°ซ) และมีเสถียรภาพเป็นอย่างน้อย 18 เดือนภายใต้เงื่อนไขเหล่านี้. During shipment, crystals or precipitation may form in the Buffer RLC. Dissolve by warming buffer to 37°C.