HiPure Gel DNA Micro Kit

HiPure Gel Pure DNA Kit uses proprietary chemistry and HiPure technology to recover DNA fragments between 60bp-10kbp with yields exceeding 80%. DNA is suitable for ligations, พีซีอาร์, การเรียงลำดับ, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.

รายละเอียด

ข้อมูลจำเพาะ

หลักการ

The HiPure system uses a simple bind-wash-elute procedure. Gel slices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.

ข้อดี

• High recovery efficiency – จนถึง 80% DNA recovery
• Low elution volume – elution volume can be as low as 10μl
• ทั่วไป – recover DNA fromgel or enzyme-driven reaction solutions such as PCR
• Fast – isolation can be completed in 10-15 minutes by column gel method

เนื้อหาชุด

การจัดเก็บและความเสถียร

The Kit should be stored dry at room temperature (15-25องศาเซลเซียส) และมีเสถียรภาพเป็นอย่างน้อย 18 เดือนภายใต้เงื่อนไขเหล่านี้. If any precipitates form in the buffers, warm at 37℃ to dissolve.