การแนะนำ

Hipure viral RNA kit is suitable for purifying viral RNA from samples such as cell-free body fluid or culture medium. The kit is based on silica gel column purification technology. It requires no toxic phenol chloroform extraction and time-consuming alcohol precipitation in the extraction. The whole extraction process takes only 25 นาที. The kit is suitable for extracting viral RNA from 1-140µl cell-free fluid samples such as serum, พลาสมา, cell-free body fluid or culture medium. The product has successfully extracted hepatitis B A/C, hepatitis C RNA, SARS and HIV. The obtained RNA can be directly used for RT-พีซีอาร์, Northern hybridization and virus detection.

รายละเอียด

ข้อมูลจำเพาะ

หลักการ

Hipure silica gel column is based on glass fiber filter membrane with high binding force. Under the condition of high concentration of ionizing agent (เช่น guanidine hydrochloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bond and electrostatic, while protein and other impurities are not adsorbed and removed. The filter membrane adsorbed with nucleic acid is washed to remove the residual protein and salt. ในที่สุด, the nucleic acid adsorbed on the filter membrane can be washed out with low salt buffer (such as buffer TE) or water. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.

The sample is homogenized and lysed in the lysate, and RNA is released into the lysate. The high concentration of guanidine isothiocyanate contained in the lysate denatured and inactivated endogenous or exogenous RNase, while RNA is protected from degradation. The lysate is centrifuged to remove insoluble impurities. After adding ethanol to adjust the binding conditions, it is transferred to the column for filtration. RNA is adsorbed on the membrane of the column, while protein is removed without adsorption. The column is washed with buffer VHB to remove protein and other impurities, washed with buffer RW2 to remove salt. Finally the RNA is eluted by RNase free water. The eluted RNA can be directly used in RT-PCR, Northern blot, poly-A purification, in vitro translation, ฯลฯ.

ข้อดี

• เร็ว – สามารถดึงตัวอย่างออกมาได้หลายตัวอย่าง 20 minutes by column method
• High quality – high purity total RNA can be directly used in various sensitive downstream applications
• ปลอดภัย – no phenol chloroform extraction required
• Sensitive – RNA can be recovered at the level of PG
• ให้ผลผลิตสูง – carrier RNA contained in the product maximize the recovery of trace nucleic acid

เนื้อหาชุด

การจัดเก็บและความเสถียร

Carrier RNA should be stored at 2–8°C upon arrival. อย่างไรก็ตาม, การจัดเก็บระยะสั้น (จนถึง 12 สัปดาห์) ที่อุณหภูมิห้อง (15–25°ซ) ไม่ส่งผลกระทบต่อประสิทธิภาพการทำงานของพวกเขา. ส่วนประกอบชุดอุปกรณ์ที่เหลือสามารถเก็บไว้ที่อุณหภูมิห้องได้ (15–25°ซ) และมีเสถียรภาพเป็นอย่างน้อย 18 months under theseconditions