ภาพรวมผลิตภัณฑ์
- Genomic DNA Reverse Transcription Kit:
- Available in M-MLV (RNase H-) Reverse Transcriptase.
- New reverse transcriptase obtained through multiple point mutations, greatly improving thermal stability and cDNA synthesis efficiency.
- Suitable for two-step RT-qPCR detection.
- RNA Template Preparation:
- Use 4xgDNA Clear Short-term treatment with Mix to completely remove residual genomic DNA contamination from RNA templates.
- Ensures subsequent quantitative results are more reliable and simplifies qPCR primer design.
- Reverse Transcription Process:
- Join 5xqRT directly Reverse Mix for immediate reverse transcription.
- 5xqRT Reverse Mix contains all components required for reverse transcription (กันชน, dNTP Mix, Hiscript Reverse Transcriptase, RNase inhibitor, Random primers/Oligo dT primer mix).
- 4xgDNA Clear Mix terminates gDNA while preserving cDNA integrity.
- RNA template volume can be added up to 60% of the total reaction volume, suitable for low-concentration RNA templates.
- Both 4xgDNA Clear Mix and 5xqRT Reverse Mix are stable at -20°C for easy storage and use.
- Optimization for qPCR:
- Specially optimized for qPCR with optimized random primers/Oligo dT primer mix ratio.
- Allows cDNA synthesis initiation from various regions of RNA transcripts, maximizing qPCR result reproducibility.
- Compatible with ซีบีอาร์ Green and probe method qPCR, allowing selection based on experimental needs.
- Reagents can be used together with SYBR Green Master Mix for high-performance gene expression analysis.
Pre-experiment preparation and important precautions:
- RNase free of 1 . 5 ml microcentrifuge tube or 0.2 มล พีซีอาร์ tube
- Water bath or เครื่องพีซีอาร์
- Ice
- อาร์เอ็นเอ: High-quality intact RNA is crucial to obtain high-quality cDNA, please use electrophoresis to verify the integrity of RNA.
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