โซลาร์บิโอ คูแมสซี (แบรดฟอร์ด) ชุดทดสอบโปรตีน 50T

Cat No.: PC0015

ขนาด: 50ต

พื้นที่จัดเก็บ: Seal preservation after opening, this kit from the date of order is valid for nine months.

คำอธิบาย

Coomassie G-250 dye is a colorimetric reagent used for the detection and quantitation of total protein. In an acidic medium Coomassie dye binds protein causing an immediate shift in absorption maximum from 465nm to 595nm with a concomitant color change from green to blue. In a certain concentration range, the absorbance value A595 measured is in direct proportion to the protein concentration.

มาตรการ

• ไมโครเพลทผู้อ่าน
  1. Completely dissolve BSA standard, dilute 10ul to 250ul, the final concentration of 0.2mg/ml. The dilution buffer was dependent on the measured protein sample. For the sake of simplicity, suggested to use 0.9%NaCl or PBS.
  2. 5×G250mix well before 1ml 5×G250 diluted with 4ml ddH2O. 1×G250 solution can save one week at 4℃.
  3. The standard according to 0, 2, 4, 6, 8, 12, 16, and 20ul respectively added to 96well plates, and PBS dilution to complement.
  4. Dilute the sample(prepare a few gradients, เช่น 2 ครั้ง, 4 ครั้ง, 8 การเจือจางครั้ง), and add 20ul sample to 96 ดี. To avoid errors, sample points need set after the standard line of 1/2.
  5. Add 200ul diluted 1×G250 to each well, room temperature for 3-5 นาที.
  6. Determination of absorbance.
  7. According to the standard curve calculate the sample protein

• UV-Visสเปกโตรโฟโตมิเตอร์

1. 8*10ml centrifuge tube(หรือมากกว่านั้น) and labeled with1~8.
2. Dilute 100ul BSA with 2.4ml PBS to 2mg/ml.
3. 5×G250 mix well before use. 1ml 5×G250 diluted with 4ml ddH2O. 1×G250 solution can save one week at4℃.
4. Add reagents according to the following table (each well 5ml, the extra is used to clean the cuvette)

5. Measure OD value after 3 minutes of reaction. To ensure the accuracy of the experiment, measure the OD value every 2 นาที

สินค้าที่เกี่ยวข้อง:

PC0001 สารละลายมาตรฐานโปรตีน (5มก./มล. บีเอสเอ) PC0021 รีเอเจนต์ BCA

PC0030 Lowry Protein Assay Kit PC0020 บีซีเอ โปรตีน Assay Kit R0010 RIPA buffer(สูง)

PR1600 พรีสเตนโปรตีนมาร์กเกอร์(14.4kD-97.4kD) R0050 Nuclear Protein Extraction Kit

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• Zhongyuan Li,Xiumei Li,Tianhui Liu,และคณะ. The critical roles of exposed surface residues for the thermostability and halotolerance of a novel GH11 xylanase from the metagenomic library of saline-alkaline soil. International Journal of Biological Macromolecu Les. JuLy 2019;133:316-323. (IF784)
• Qinlu Zhang,Qian Liu,Menghan Du,และคณะ. Cetuximab and Doxorubicin loaded dextran-coated Fe3O4 magnetic nanoparticles as novel targeted nanocarriers for non-small cell lung cancer. Journal of Magnetism and Magnetic Materials. June 2018. (IF046)