bird-dna-sexing
For bird breeders, especially large-scale breeding farms for 鸚鵡, pigeons, ETC。, the construction of a small molecular detection laboratory can quickly detect the sex of fledglings by detecting genes, and can also detect bird viruses during the breeding process and achieve early prevention.
The construction of a small DNA detection laboratory does not require a large investment in laboratory construction, but a relatively clean space is sufficient. The instruments and 裝置 required for a small DNA detection laboratory are the most basic and necessary, and the quantity is not large.
這裡, I have compiled a list of the equipment and reagents needed to build a small-scale avian DNA detection laboratory, along with reference prices.
| 即時的 聚合酶鍊式反應 method to detect the sex identification protocol of parrots, pigeons, and jade birds – SanshiBio ( price for entire system only) | |||||
| 不. | 產品名稱 | 模型 | Price($) | 數量 | Remark |
| 1 | Real-time fluorescent quantitative PCR | X4 | 18286 | 1 | 4 頻道 、96 井 |
| 2 | Rapid 基因組DNA萃取 Kit forAnimals (用於PCR分析) | 200時間 | 50 | 1 | |
| 3 | Gender detection reagent | 100時間 | 278 | 2 | |
| 4 | Mini centrifuge | MINI10K+C | 214 | 1 | |
| 5 | Mini mixer | MIX-25P | 214 | 1 | |
| 6 | 吸管 | 10ul | 29 | 1 | |
| 7 | 吸管 | 100ul | 29 | 1 | |
| 8 | 吸管 | 1000ul | 29 | 1 | |
| 9 | 移液器吸頭 | 10ul | 2 | 1 | |
| 10 | 移液器吸頭 | 100ul | 2 | 1 | |
| 11 | 移液器吸頭 | 1000ul | 3 | 1 | |
| 12 | 0.2ml eight-row tube | 0.2毫升 | 34 | 1 | |
| 13 | 2.0ml centrifuge tubes | 500 pcs | 6 | 1 | |
| 14 | Disposable latex gloves | 9 | 1 | ||
| 15 | Icebox | 0.2ml*96 | 21 | 1 | |
| 16 | 1.5ml/2.0ml EP tube rack | 1 | 1 | ||
| 17 | 0.2 ml EP tube rack | 96 井 | 5 | 5 | |
| 18 | Nucleic acid contamination scavenger NC | 500毫升 | 86 | 1 | |
| 19 | Scissors | 0 | Prepare yourself | ||
| 20 | Tweezers | 0 | Prepare yourself | ||
| 21 | Desktop trash can | 0 | Prepare yourself | ||
| 22 | Disposable garbage bags | 0 | Prepare yourself | ||
| 23 | Disinfect alcohol | 0 | Prepare yourself | ||
| 24 | paper towel | 0 | Prepare yourself | ||
| 25 | Cryogenic freezer | 0 | Prepare yourself | ||
| Optional Instrument | |||||
| 1 | Hot lid metal bath | DTC-100H | 952 | ||
| 2 | Hot lid metal bath | DTH-100H | 640 | ||
As we can see from the list, the most expensive part is the “Real-time fluorescent quantitative PCR機」. 然而, if you do not have enough budget and you do not want to test 1000 birds a day, you may choose a smaller qPCR machine that is much cheaper less than 10k USD.
For avian detection laboratories, a large area is not required for construction. 一般來說, a maximum of about 30 square meters is sufficient. If the sample size for detection is small, a smaller space can be used.
At least one person is required for avian gender test experiments. Efficiency increases with two people; one can handle sample preparation while the other prepares for the detection process.
定量PCR bird gender and disease test experiments do not have very high cleanliness requirements. Daily cleanliness, minimal dust, and dry conditions are sufficient.
Yes, other brands of detection equipment can be used as long as they are operated according to the requirements of the detection reagents.
In one avian gender detection, the processing time for one set of samples (96時間) is approximately 30 分分鐘, while qPCR detection from preparation to result output takes about 40-50 分分鐘.
A set of samples can accommodate up to 96 樣品, with a general recommendation of 90 樣品. Smaller channel devices can typically test around 48 樣品.
Besides avian gender testing, a complete set of PCR detection equipment allows for various other test experiments using different reagents.
Yes, the company provides comprehensive laboratory operation training. 另外, they welcome on-site learning in China, which typically takes 2-3 天. Guidance is also available via direct video communication.
Here is guidance on laboratory equipment and reagent operations, including instructional videos for reference and learning：
https://drive.google.com/drive/folders/14HkhVCirVzbfKqGlOopRt-jkOPLhvsum?usp=drive_link
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