介紹
- Guanidine hydrochloride is a crucial component in nucleic acid extraction, selected after stringent screening and quality control.
- It acts as a powerful denaturant and ionizing agent, enhancing the solubility of hydrophobic molecules and denaturing proteins.
- Guanidine hydrochloride is effective in denaturing and inactivating various enzymes and proteins.
- In a 6 M鹽酸胍溶液, 這 “二級結構” of proteins, maintained by intermolecular non-covalent interactions, is disrupted, causing changes in the tertiary structure.
- It is commonly used in nucleic acid extraction as a cleaning solution (> 3中號), 結合溶液 (4-6中號) 用於去除蛋白質, 基因組 DNA 和總 DNA 的裂解液 RNA萃取.
- Unlike guanidine isothiocyanate, guanidine hydrochloride exhibits low absorbance in A260, A280, and A230, resulting in an ideal ratio of nucleic acid product A260/A230.
規格
| 特徵 | 規格 | |
| 基本內容 | 推薦應用 | 質粒萃取, lysis Solution, or cleaning solution |
| CAS 編號 | 50-01-1 | |
| 公式 | CH5N3鹽酸鹽 | |
| 分子量 | 95.53 | |
| 內容 | 99.5% | |
| 等級 | 分子生物學 | |
| 外貌 | White crystal will harden for a long time | |
| 運輸條件 | 室內溫度 | |
| 保存條件 | 室內溫度, 乾燥, 黑暗的 | |
| 雜質參數 | 水分 | ≤0.3% |
| 灰 | ≤0.02% | |
| 水不溶物 | ≤0.02% | |
| 銨 | ≤0.05% | |
| 二氰化銨 | ≤0.1% | |
| 氰尿酰氯化銨 | ≤0.01% | |
| 鐵 | ≤0.1PPM | |
| 紫外線吸收值 | 吸光度值@ 230 (6中號) | ≤0.4 |
| 吸光度值@ 260 (6中號) | ≤0.04 | |
| 吸光度值@ 280 (6中號) | ≤0.01 | |
| 吸光度值@ 320 (6中號) | ≤0.01 | |
| 核酸萃取相關 | 質粒萃取試驗 | 採納 |
| 血液DNA提取測試 | 採納 | |
| 脫氧核糖核酸酶測試 (2中號) | 沒有檢測到 | |
| 核糖核酸酶測試 (2中號) | 沒有檢測到 | |
| 飽和溶液的純度 (8中號) | 8m母液加熱溶解. 解散後, 過濾不溶物 3 快速濾紙層數. | |
| PH值 (6中號) | 5.0-5.5 |
訂購資訊
| 目錄號. | 產品名稱 | 包裹 |
| C11101 | 鹽酸胍 (分子生物學) | 5公斤 |
| C11102 | 25公斤 (5公斤/袋, 5袋/桶) |
評論
還沒有評論.