介紹
該產品是經過嚴格篩選和品質控制的核酸萃取核心原料. Guanidine thiocyanate is commonly used as lysate denaturant, and agarose gel sol in the process of nucleic acid extraction. Large 2m guanidine isothiocyanate can mediate the adsorption of isodna on glass fiber filter membranes or silica magnetic beads. Different from guanidine hydrochloride, guanidine isothiocyanate has a high absorbance value in A230, 0.5% (w / V) guanidine isothiocyanate (~4mm), and its A260 / A230 is less than 0.5. For the nucleic acid obtained by using this lysate, its A260 / 230 will be poor in low-concentration nucleic acid samples, but it will not affect 聚合酶鍊式反應, 逆轉錄聚合酶連鎖反應, and second-generation sequencing.
細節
規格
| 特徵 | 規格 | |
| 基本內容 | 推薦應用 | Agarose Gel DNA純化, DNA/RNA Isolation (Lysis Buffer) |
| CAS 編號 | 593-84-0 | |
| 公式 | CH5N3•HSCN | |
| 分子量 | 118.6 | |
| 內容 | 99.5% | |
| 等級 | 分子生物學 | |
| 外貌 | White powder to crystal | |
| 運輸條件 | 室內溫度, 乾燥 | |
| 保存條件 | Avoid light | |
| 雜質參數 | 水分 | ≤0.3% |
| 灰 | ≤0.02% | |
| 水不溶物 | ≤0.02% | |
| 銨 | ≤0.05% | |
| 二氰化銨 | ≤0.1% | |
| 氰尿酰氯化銨 | ≤0.01% | |
| 鐵 | ≤0.1PPM | |
| 紫外線吸收值 | 吸光度值@ 230 (6中號) | ≤20 |
| 吸光度值@ 260 (6中號) | ≤25 | |
| 吸光度值@ 280 (6中號) | ≤0.3 | |
| 吸光度值@ 320 (6中號) | ≤0.01 | |
| 核酸萃取相關 | Gel DNA Purification | 採納 |
| RNA Isolation | 採納 | |
| 脫氧核糖核酸酶測試 (1中號) | 沒有檢測到 | |
| 核糖核酸酶測試 (1中號) | 沒有檢測到 | |
| 飽和溶液的純度 (6中號) | Endothermic during dissolution, no precipitation at room temperature after heating and dissolution, clear and colorless (no filtration) | |
| PH值 (1中號) | 5.0-7.0 |
訂購資訊
| 目錄號. | 產品名稱 | 包裹 |
| C11103 | Guanidine Thiocyanate (分子生物學) | 5公斤 |
| C11104 | 25公斤 (5公斤/袋, 5袋/桶) |
評論
還沒有評論.