This product provides fast and easy methods for purification of total DNA for reliable 聚合酶鍊式反應 and southern blotting. Total DNA can be purified from bacterial culture, 體液, food and fermentation.
細節
規格
| 特徵 | 規格 |
| 主要功能 | Isolation bacterial DNA from cultures, food and other samples |
| 應用領域 | 聚合酶鍊式反應, southern blot and enzyme digestion, ETC. |
| 純化方法 | 小型的 旋轉柱 |
| 淨化技術 | 二氧化矽技術 |
| 工藝方法 | 手動的 (離心或真空) |
| 樣品類型 | Culture medium, swab, parasitic blood, 組織,sputum, ETC. |
| 樣品量 | Bacterial culture medium: 0.5-2 毫升 組織樣本: 50-100 毫克 全血 / 細胞懸浮液: 0.5-1 毫升 Viscous secretion: 0.1-1 G |
| 洗脫體積 | ≥30μl |
| 每次運行時間 | ≤40分鐘 |
| 每根柱的載液量 | 800微升 |
| 柱結合率 | 100微克 |
原則
本產品以矽膠柱純化. 用裂解物和蛋白酶裂解和消化樣品, DNA 釋放到裂解液中. 轉移至吸附柱. 核酸吸附在膜上, while proteinis not adsorbed and is removed with filtration. 洗滌蛋白質及其他雜質後, 最後用低鹽緩衝液洗脫核酸 (10mmTris, pH9.0, 0.5乙二胺四乙酸). This product carries 0.1-0.2mm acid glass beads, which can improve the wall-breaking effect of lysozyme resistant bacteria, the success rate and DNA yield through physical bead grinding.
優點
- 快速地 – 可以提取多個樣本 40 分分鐘 (after digestion)
- 高純度 – purified DNA can be directly used in various downstream applications
- Good repeatability – silica technology can obtain ideal results every time
- 高回收率 – DNA can be recovered at the level of PG
- Broad spectrum – it can deal with a variety of bacteria, including Gram-positive bacteria that are difficult to disrupt
- Sufficient components – this kit has carried lysozyme, protease K, 核糖核酸酶A and glass beads
套件內容
| 內容 | D314602 | D314603 |
| 淨化時間 | 50 準備工作 | 250 準備工作 |
| Hipure DNA Mini Columns I | 50 | 2 X 125 |
| 2毫升收集管 | 50 | 2 X 125 |
| Glass Beads (0.1~0.2mm) | 20 G | 100 G |
| Buffer P1 | 20 毫升 | 100 毫升 |
| Buffer DL | 15 毫升 | 80 毫升 |
| 緩衝器GW1 | 22 毫升 | 88 毫升 |
| 緩衝器GW2 | 12 毫升 | 50 毫升 |
| 溶菌酶 | 60 毫克 | 300 毫克 |
| 蛋白酶K | 24 毫克 | 120 毫克 |
| 蛋白酶溶解緩衝液 | 5 毫升 | 15 毫升 |
| Buffer AE | 15 毫升 | 60 毫升 |
儲存和穩定性
Lysozyme and Proteinase K should be stored at 2-8°C upon arrival. 然而, 短期儲存 (取決於 12 週) 在室溫下 (15-25℃) 不影響他們的表現. 其餘套件組件可在室溫下乾燥保存 (15-25℃) 並且至少穩定 18 在這些條件下幾個月.
評論
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