介紹
HiPure Bacterial RNA Kit uses silica gel column purification to simplify the extraction. The whole process does not require phenol chloroform extraction and time-consuming alcohol precipitation. The kit is suitable for efficiently extracting RNA from various bacterial samples. The purified RNA can be directly used for RT-聚合酶鍊式反應, 北方雜交, ETC. The kit has included lysozyme and glass beads, which can be used to treat gram-negative bacteria which is easy to be lysed, as well as gram-positive bacteria which is hard to be lysed, including enterococcus faecalis, staphylococcus, ETC.
細節
規格
| 特徵 | 規格 |
| 主要功能 | Isolation total RNA from bacteria culture |
| 應用領域 | 逆轉錄聚合酶連鎖反應, Northern blot, poly-A + purification, 核酸保護與體外翻譯 |
| 純化方法 | 小型的 旋轉柱 |
| 淨化技術 | 二氧化矽技術 |
| 工藝方法 | 手動的 (離心或真空) |
| 樣品類型 | Bacteria culture |
| 樣品量 | 細菌: <10^9 |
| 洗脫體積 | ≥30μl |
| 每次運行時間 | ≤40分鐘 |
| 每根柱的載液量 | 800微升 |
| 柱結合率 | 100微克 |
原則
Hipure矽膠柱以高結合力為基礎的玻璃纖維濾膜. 高濃度電離劑條件下 (例如 胍 hydrochloride or guanidine isothiocyanate), 濾膜可透過氫鍵和靜電吸附核酸, while protein and other impurities are not adsorbed and removed. 清洗吸附有核酸的濾膜，去除殘留的蛋白質和鹽分. 最後, 吸附在濾膜上的核酸可以用低鹽緩衝液洗掉 (例如緩衝液TE) 或水. 所得的核酸純度高，可直接用於各種下游實驗.
優點
- 快速地 – 可以提取多個樣本 30 分分鐘
- 高純度 – the purified RNA can be directly used in various downstream applications
- 高回收率 – RNA can be recovered at the level of PG
- Good repeatability – silica gel column purification technology can obtain ideal results every time
- Broad spectrum – it can deal with various bacteria, including Gram-positive bacteria that are difficult to be lysed
- Sufficient components – the kit contains carried lysozyme and glass beads
套件內容
| 內容 | R418102 | R418103 |
| 淨化時間 | 50 準備工作 | 250 準備工作 |
| HiPure RNA 迷你柱 | 50 | 250 |
| 2毫升收集管 | 50 | 250 |
| Glass Beads (0.1-0.2毫米) | 20 G | 90 G |
| 溶菌酶 | 90 毫克 | 400 毫克 |
| 蛋白酶溶解緩衝液 | 5 毫升 | 15 毫升 |
| Buffer STE | 10 毫升 | 30 毫升 |
| MagZol試劑 | 60 毫升 | 270 毫升 |
| 緩衝器 RW2* | 20 毫升 | 2 X 50 毫升 |
| 無核糖核酸酶水 | 10 毫升 | 30 毫升 |
儲存和穩定性
MagZol Reagent and Lysozyme should be stored at 2-8°C upon arrival. 其餘套件組件可在室溫下乾燥保存 (15-25℃) 並且至少穩定 18 在這些條件下幾個月. 整個套件可在 2-8°C 下保存, 但在這種情況下，緩衝液應在使用前重新溶解. 使用時確保所有緩衝液均處於室溫.
評論
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