The Kit provides fast purification of high-quality RNA from whole blood, 細胞, and tissues using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or isopropanol precipitation. The purified RNA can be used for applications such as RT-聚合酶鍊式反應, 北方印跡, poly A+ RNA (信使RNA) purification, nuclease protection, and in vitro translation.
細節
規格
| 特徵 | 規格 |
| 主要功能 | Isolation of total RNA from 1-1.5ml whole blood |
| 應用領域 | 定量PCR / 逆轉錄聚合酶連鎖反應, liquid or solid-phase chip analysis, 雜交, and SNP detection |
| 純化方法 | 小型的 旋轉柱 |
| 淨化技術 | 二氧化矽技術, acid phenol/胍 extraction technology (MagZol預處理技術) |
| 工藝方法 | 手動的 (離心或真空) |
| 樣品類型 | Fresh or frozen blood, mammalian blood |
| 樣品量 | ≤1.5 ml whole blood |
| 屈服 | 2-100微克 |
| 洗脫體積 | ≥20μl |
| 每根柱的載液量 | 800微升 |
| 柱結合率 | 100微克 |
原則
The Kit simplifies the isolation of RNA from blood with a fast spin-column procedure. Red blood cells are selectively lysed and white cells are collected by centrifugation. White cells are then lysed using highly denaturing conditions which immediately inactivate RNases. After homogenization using the DNA spin column, the sample is applied to the RNA column. Total RNA binds to the membrane and contaminants are washed away, leaving pure RNA to be eluted in 30–100µl RNase-free water (provided with the kit) for direct use in any downstream application.
優點
- 高品質 – high purity total RNA can be directly used in various sensitive downstream applications
- 快速地 – isolation of several samples can be completed in 20 minutes by using the column purification method
- 敏感的 – RNA can be purified at the level of PG
套件內容
| 內容 | R416102 | R416103 |
| 淨化時間 | 50 準備工作 | 250 準備工作 |
| HiPure DNA Mini Columns | 50 | 250 |
| HiPure RNA Mini Columns I | 50 | 250 |
| 2毫升收集管 | 100 | 500 |
| 10 x Buffer RBC | 50 毫升 | 3 X 100 毫升 |
| RTL Lysis Buffer | 50 毫升 | 250 毫升 |
| 緩衝器RW1 | 50 毫升 | 250 毫升 |
| 緩衝器 RW2* | 20 毫升 | 2 X 50 毫升 |
| 無核糖核酸酶水 | 10 毫升 | 30 毫升 |
儲存和穩定性
HiPure Blood RNA Mini Kit components can be stored dry at room temperature (15-25℃) 並且至少穩定 18 在這些條件下幾個月. 使用時確保所有緩衝液均處於室溫. During shipment, crystals or precipitation may form in the RTL Lysis Buffer. Dissolve by warming buffer to 37°C.
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