HiPure Circulating DNA Midi Kit, designed for efficient purification of short, 遊離DNA (遊離DNA) molecules from human biofluids for clinical use:
- 目標:
- 血液中腫瘤來源的 cfDNA 片段和 mRNA
- 母血中的胎兒核酸
- 樣品相容性:
- 人類血漿
- 人血清
- 人類尿
- 樣品可以是新鮮的或冷凍的 (僅一次)
- 專注於短DNA: 該試劑盒分離短核酸 (<1000BP) 常見於生物體液中.
- 高效淨化: 此方案可有效從各種生物液中回收 cfDNA.
- Clinical Applications: The purified cfDNA is suitable for use in in vitro diagnostic (體外診斷) tests approved for clinical purposes.
好處:
- 針對短 cfDNA 進行了優化: 專門設計用於捕獲與生物標記發現和液體活檢相關的短 DNA 片段.
- 廣泛的樣品相容性: 與各種人體生物體液有效配合, 提供樣本選擇的靈活性.
- Larger Scale Purification: The Midi Kit format allows for processing of larger sample volumes compared to Mini Kits, potentially increasing cfDNA yield.
- Clinical Utility: Purified DNA can be directly used in approved clinical IVD tests.
This kit empowers researchers and clinical laboratories studying circulating DNA for applications in oncology, 非侵入性產前檢測 (NIPT), and liquid biopsy analyses, ultimately aiding in patient diagnosis and management.
規格
| 特徵 | 規格 |
| 主要功能 | 從 1-5ml 血漿中分離循環 DNA, 血清, 使用真空協議提取體液 |
| 應用領域 | 定量PCR, 液體或固體晶片分析, 雜交和SNP檢測, ETC. |
| 純化方法 | 小型的 旋轉柱 |
| 淨化技術 | 二氧化矽技術 |
| 工藝方法 | 手動的 (真空) |
| 樣品類型 | 血清, 血漿和其他無細胞液體樣本 |
| 樣品量 | 1-5毫升 |
| 洗脫體積 | ≥50μl |
| 每次運行時間 | ≤60分鐘 |
| 每根柱的載液量 | 4毫升 |
| 柱結合率 | 1毫克 |
原則
This product offers a fast and reliable method for purifying DNA using silica column chromatography.
- 裂解和消化: The sample is treated with a solution and enzyme to break open cells and degrade proteins, releasing DNA.
- Binding to Silica: The lysate is transferred to a column. The silica membrane in the column captures DNA molecules, while impurities pass through.
- 洗滌: Wash buffers to remove unbound proteins and cellular debris.
- 洗脫: A low-salt buffer elutes the purified DNA from the silica.
此方法為需要純化 DNA 進行分析的各個領域的研究人員提供了寶貴的工具.
優點
- 高產 – 最優過程, 遊離DNA (>50BP) 可以最大程度地獲得
- 高濃度 – 低洗脫體積, 確保高核酸濃度
- 高純度 – 低醇結合法, 徹底去除抑制劑和蛋白質污染
- 高回收率 – 透過矽膠柱純化可在PG水平回收DNA
套件內容
| 內容 | D318201C | D318202C |
| 淨化時間 | 10 | 50 |
| 緩衝區ACL | 50 毫升 | 250 毫升 |
| 緩衝器 ACB* | 60 毫升 | 300 毫升 |
| 緩衝器 DCW1* | 4.4 毫升 | 22 毫升 |
| 緩衝器 DCW2* | 5 毫升 | 10 毫升 |
| 蛋白酶K | 110 毫升 | 540 毫克 |
| 蛋白酶溶解緩衝液 | 10 毫升 | 30 毫升 |
| 載體RNA | 110 微克 | 110 微克 |
| 無核酸酶水 | 10 毫升 | 20 毫升 |
| HiPure CFDNA 迷你柱 | 10 | 50 |
| 2 毫升收集管 | 20 | 100 |
| 延長管 | 10 | 50 |
| 真空連接器 | 10 | 50 |
儲存和穩定性
- Keep it cool: Store Proteinase K and Carrier RNA in the fridge (2-8℃) for best results.
- Room temp is okay (for a while): They can last at room temperature (15-25℃) 最多為 12 weeks without issues.
- Everything else: Store other kit components dry at room temperature (15-25℃), good for at least 18 月.
- Important: If storing everything cold, remember to warm up the buffers before use.
實驗數據
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