產品概述
- Offers fast and easy methods for purifying total DNA.
- Ensures reliable 聚合酶鍊式反應 and Southern blotting results.
- Capable of purifying various types of DNA, including genomic, viral, and mitochondrial.
- Can purify DNA from small volumes of blood, 組織, and dry blood spots.
規格
| 特徵 | 規格 |
| 主要功能 | Isolation total DNA from 1-10μl blood, <10mg tissue, urine, blood stain, seminal stain |
| 應用領域 | 聚合酶鍊式反應, 南方螺栓和病毒檢測, ETC. |
| 純化方法 | 小型的 旋轉柱 |
| 淨化技術 | 二氧化矽技術 |
| 工藝方法 | 手動的 (離心或真空) |
| 樣品類型 | 動物組織, blood stain, urine, seminal stain and various forensic samples |
| 樣品量 | 血:1-100微升, 組織:<10毫克 |
| 洗脫體積 | |
| 每次運行時間 | |
| 每根柱的載液量 | |
| 柱結合率 |
原則
- Utilizes silica column purification method.
- Sample is lysed and digested with lysate and protease, releasing DNA into the lysate.
- Lysate is transferred to an adsorption column.
- 核酸吸附在膜上, 而蛋白質不被吸附並透過過濾去除.
- Proteins and other impurities are washed away.
- Nucleic acid is finally eluted with low-salt buffer (10毫米三羥甲基氨基甲烷, 酸鹼度 9.0, 0.5乙二胺四乙酸).
優點
- 快速地 – 可以提取多個樣本 20 分分鐘 (after digestion)
- 高純度 – purified DNA can be directly used in various downstream applications
- 高回收率 – DNA can be recovered at the level of PG
- Good repeatability – silica technology can obtain ideal results every time
套件內容
| 內容 | D312502 | D312503 |
| 淨化時間 | 50 準備工作 | 250 準備工作 |
| 緩衝區ATL | 15 毫升 | 60 毫升 |
| 緩衝液AL | 15 毫升 | 60 毫升 |
| Buffer GW1* | 22 毫升 | 66 毫升 |
| Buffer GW2* | 20 毫升 | 2 X 50 毫升 |
| 載體RNA | 310 微克 | 2 X 310 微克 |
| 蛋白酶K | 24 毫克 | 120 毫克 |
| 蛋白酶溶解緩衝液 | 1.8 毫升 | 10 毫升 |
| Buffer AE | 15 毫升 | 60 毫升 |
| HiPure DNA 迷你柱 I | 50 | 2 X 125 |
| 2 毫升收集管 | 100 | 5 X 100 |
儲存和穩定性
- Carrier RNA and Proteinase K should be stored at 2–8°C upon arrival.
- Short-term storage at room temperature (15–25°C) 最多為 12 weeks does not affect their performance.
- Remaining kit components can be stored dry at room temperature (15–25°C) and remain stable for at least 18 在這些條件下幾個月.
實驗數據
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