介紹
The combination of improved CTAB extraction method and column purification technology is suitable for extracting high-purity RNA from various plant/fungal samples, especially plant tissues rich in polysaccharides and polyphenols (such as cotton leaves, mature rice leaves, Arabidopsis seeds, white pine needles, loquat leaves, roses, ETC。). The obtained RNA can be directly used for experiments such as RT-聚合酶鍊式反應, 印跡雜交, 多聚A+純化, nucleic acid protection, and in vitro translation. This product does not contain 胍 isothiocyanate throughout the entire process, and the purified RNA product does not contain guanidine isothiocyanate contamination, making A260/230 more stable.
細節
套件內容
內容 | R4156 |
淨化時間 | 100 準備工作 |
Column B10 | 2 X 100 |
2毫升收集管 | 2 X 100 |
Buffer PAL | 100 毫升 |
Buffer PRC1 | 60 毫升 |
Buffer SW1 | 60 毫升 |
Buffer SW2 | 110 毫升 |
無核糖核酸酶水 | 20 毫升 |
儲存和穩定性
The kit components can be stored at room temperature (15-25℃) 並且至少穩定 18 月.
評論
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