介紹
HiPure Insect DNA Kits provides a simple and rapid solution for total DNA extraction of insect tissue samples. This kit is based on silica gel column purification technology without toxic phenol chloroform extraction and time-consuming alcohol precipitation. The whole extraction process only takes 30 分分鐘. HiPure Insect DNA Kit can process tissue samples less than 10mg at a time. Hipure Insect DNA 96 kit can process 96 insect tissue samples at a high throughput. The obtained DNA can be directly used in 聚合酶鍊式反應, Southern blot, viral DNA detection and other experiments.
細節
規格
| 特徵 | 規格 |
| 主要功能 | Isolation total DNA from insect tissue |
| 應用領域 | 聚合酶鍊式反應, 南方螺栓和病毒檢測, ETC |
| 純化方法 | 96 well DNA plate |
| 淨化技術 | 二氧化矽技術 |
| 工藝方法 | 手動的 (離心或真空) |
| 樣品類型 | Insect tissue samples |
| 樣品量 | |
| 洗脫體積 | |
| 每次運行時間 | |
| 每根柱的載液量 | |
| 柱結合率 |
Principles
This product is based on silica column purification. 用裂解物和蛋白酶裂解和消化樣品, DNA 釋放到裂解液中. 轉移至吸附柱. 核酸吸附在膜上, 而蛋白質不被吸附並透過過濾去除. 洗滌蛋白質及其他雜質後, 最後用低鹽緩衝液洗脫核酸 (10毫米三羥甲基氨基甲烷, pH9.0, 0.5乙二胺四乙酸).
優點
- High purity DNA – can be used in sensitive downstream applications such as multiplex and quantitative pcr
- Good repeatability – suitable for extracting high-yield DNA from insect tissue samples
- 安全的 – without phenol chloroform extraction and alcohol precipitation
套件內容
| 內容 | D313901 | D313902 |
| 淨化時間 | 1 X 96 | 4 X 96 |
| HiPure DNA Plate | 1 | 4 |
| 2.2 ml Collection Plate | 1 | 4 |
| 1.6 ml Collection Plate | 1 | 4 |
| 0.5ml Collection Plate | 1 | 4 |
| Seal Film | 8 | 32 |
| Buffer ITL | 30 毫升 | 120 毫升 |
| Buffer IL | 30 毫升 | 125 毫升 |
| 緩衝器GW1 | 44 毫升 | 2 X 110 毫升 |
| 緩衝器GW2 | 50 毫升 | 3 X 50 毫升 |
| 蛋白酶K | 50 毫克 | 200 毫克 |
| 蛋白酶溶解緩衝液 | 6 毫升 | 15 毫升 |
| Buffer AE | 20 毫升 | 60 毫升 |
儲存和穩定性
蛋白酶K, 核糖核酸酶A should be stored at 2-8°C upon arrival. 然而, 短期儲存 (取決於 12 週) 在室溫下 (15-25℃) 不影響他們的表現. 其餘試劑盒成分可在室溫下保存 (15-25℃) 並且至少穩定 18 在這些條件下幾個月. The entire kit can be stored at 2–8°C, but in thiscase buffers should be redissolved before use. Make sure that all buffers areat room temperature when used.
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