This product provides an easy-to-use workflow for selective isolation of bacterial DNA from samples that are intrinsically rich in host DNA, such as body fluids or swabs. The method is specific for the identification of intact bacteria so it prevents false results due to nucleic acids from dead bacteria. The Kit allows isolation of enriched bacterial DNA suitable for a variety of applications, including qPCR and whole metagenome or 16S rRNA gene sequencing.
細節
規格
| 特徵 | 規格 |
| 主要功能 | Isolation gDNA from biological sample and remove host DNA |
| 應用領域 | 聚合酶鍊式反應, southern blot and enzyme digestion, ETC. |
| 純化方法 | 小型的 旋轉柱 |
| 淨化技術 | 二氧化矽技術 |
| 工藝方法 | 手動的 (離心或真空) |
| 樣品類型 | Culture medium, swab, parasitic blood, 組織, sputum, ETC. |
| 樣品量 | 血液樣本, 勻漿, 電漿, brain effusion, swab immersion solution, centrifuged concentrated liquid, ETC。:0.5-1毫升 |
| 洗脫體積 | ≥50μl |
| 每次運行時間 | ≤60分鐘 |
| 每根柱的載液量 | 800微升 |
| 柱結合率 | 100微克 |
原則
本產品以矽膠柱純化. This product efficiently depletes human and animal host DNA and yields enriched bacterial DNA. An optimized combination of mechanical and chemical lysis allows efficient disruption of bacterial cells. Target DNA is adsorbed on the membrane, 而蛋白質不被吸附並透過過濾去除. 洗滌蛋白質及其他雜質後, nucleic acid was finally eluted with low-salt buffer (10毫米三羥甲基氨基甲烷, pH9.0, 0.5乙二胺四乙酸).
優點
- 高濃度 – the host DNA is digested by dnase, without contamination of host DNA
- 高回收率 – DNA can be recovered at the level of PG
- Good repeatability – silica technology can obtain ideal results every time
- Wide applicability – it can be used in blood, 組織, intestinal contents and other samples
套件內容
| 內容 | D314802 | D314803 |
| 淨化時間 | 50 準備工作 | 250 準備工作 |
| Hipure DNA Mini Columns I | 50 | 2 X 125 |
| 2毫升收集管 | 50 | 2 X 125 |
| 2ml beads Tubes | 50 | 250 |
| Buffer DRB | 15 毫升 | 60 毫升 |
| Buffer ES | 6 毫升 | 30 毫升 |
| DX 試劑 | 0.5 毫升 | 1 毫升 |
| Buffer DL | 30 毫升 | 120 毫升 |
| 緩衝器GW1 | 22 毫升 | 110 毫升 |
| 緩衝器GW2 | 12 毫升 | 50 毫升 |
| 脫氧核糖核酸酶I (粉末) | 6 毫克 | 30 毫克 |
| 蛋白酶K | 24 毫克 | 120 毫克 |
| 蛋白酶溶解緩衝液 | 5 毫升 | 15 毫升 |
| Buffer AE | 15 毫升 | 60 毫升 |
儲存和穩定性
DNase I and Proteinase K should be stored at 2–8°C upon arrival. 然而, 短期儲存 (取決於 12 週) 在室溫下 (15–25°C) 不影響他們的表現. 其餘套件組件可在室溫下乾燥保存 (15–25°C) 並且至少穩定 18 在這些條件下幾個月. The entire kit can be stored at 2–8°C, 但在這種情況下，緩衝液應在使用前重新溶解. 使用時確保所有緩衝液均處於室溫.
評論
還沒有評論.