介紹

The HiPure 質粒DNA Mega Kits combine the power of HiPure technology with the time-tested consistency of alkaline-安全資料表 lysis of bacterial cells to deliver high-quality DNA. HiPure DNA columns facilitate the binding, washing and elution steps thus enabling multiple samples to be simultaneously processed. Plasmid DNA purified by this system is suitable for automated fluorescent DNA sequencing, restriction endonuclease digestion, transfection of mammalian cells, and other manipulations. 取決於 10 mg high copy number plasmid DNA can be purified from 500 mL overnight culture.

細節

規格

原則

The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method，then transfer the supernatant to column to bind DNA. 洗滌蛋白質及其他雜質後, nucleic acid was finally eluted with low-salt buffer (10毫米三羥甲基氨基甲烷, pH9.0, 0.5乙二胺四乙酸).

優點

• 高純度 – purified plasmid can be directly used in sequencing, enzyme digestion and 聚合酶鍊式反應, ETC.
• 快速地 – silica gel column purification is much faster than ion exchange
• 高產 – up to 10mg plasmid can be binded in one column
• Low endotoxin – the obtainedplasmid can be directly used for cell transfection and animal injection, ETC.

套件內容

儲存和穩定性

The kit components can be stored dry at room temperature (15-25℃) 並且至少穩定 18 在這些條件下幾個月. 如果緩衝液中形成任何沉澱物, 37℃溫熱溶解.

Purchase Guide