介紹

The HiPure Midi system provides a fast, simple, and cost-effective 質粒DNA midiprep method for routine molecular biology laboratory applications. HiPure Midiprep Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. Plasmid DNA purified with Mini Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of Tris buffer or water.

細節

規格

原則

The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method，then transfer the supernatant to column to bind DNA. 洗滌蛋白質及其他雜質後, nucleic acid was finally eluted with low-salt buffer (10毫米三羥甲基氨基甲烷, pH9.0, 0.5乙二胺四乙酸).

優點

• 高純度 – purified plasmid can be directly used in sequencing, enzyme digestion and 聚合酶鍊式反應, ETC.
• 快速地 – silica gel column purification is much faster than ion exchange
• 高產 – up to 250μg plasmid can be binded in one column
• Low endotoxin content – the obtained plasmid can be directly used in cell transfection and animal injection

套件內容

儲存和穩定性

The kit components can be stored dry at room temperature (15-25℃) 並且至少穩定 18 在這些條件下幾個月. 如果緩衝液中形成任何沉澱物, 37℃溫熱溶解. After addition of RNase A and optional LyseBlue reagent, Buffer P1 is stable for 6 months when stored at 2–8°C.

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