介紹
The kit offers the unique feature to isolate total RNA including small RNA and DNA from serum and plasma without the need to resort to the cumbersome phenol/chloroform extraction or a time consuming proteinase digest. RNA purified using the kit is ready for applications such as RT-聚合酶鍊式反應, 北方印跡, poly A+ RNA (信使RNA) purification, nuclease protection, and in vitro translation.
細節
規格
| 特徵 | 規格 |
| 主要功能 | Isolation total RNA and miRNA from 0.2-0.6ml serum and plasma |
| 應用領域 | |
| 純化方法 | 小型的 旋轉柱 |
| 淨化技術 | 二氧化矽技術 |
| 工藝方法 | 手動的 (離心或真空) |
| 樣品類型 | |
| 樣品量 | |
| 屈服 | |
| 洗脫體積 | |
| 每次運行時間 | |
| 每根柱的載液量 | |
| 柱結合率 |
原則
HiPure RNA technology simplifies total RNA isolation. The sample material is denatured in Lysis Buffer. The protein is then precipitated by Protein Precipitation Solution and pelleted by centrifugation. After the removal of protein the binding conditions for nucleic acids are adjusted by adding isopropanol. Total nucleic acids are bound to the column. Optionally, DNA can be removed by an on-column DNase digest. The remaining nucleic acids are washed and eluted with minimal amounts of RNase-Free water.
套件內容
| 內容 | R431402 | R431403 |
| 淨化時間 | 50 準備工作 | 250 準備工作 |
| HiPure RNA 迷你柱 | 100 | 2 X 250 |
| 2毫升收集管 | 100 | 2 X 250 |
| Buffer CFL | 6 毫升 | 30 毫升 |
| Buffer CFP | 1.8 毫升 | 10 毫升 |
| 緩衝區RWC | 20 毫升 | 80 毫升 |
| 緩衝器 RW2* | 20 毫升 | 2 X 50 毫升 |
| 無核糖核酸酶水 | 10 毫升 | 60 毫升 |
儲存和穩定性
The kit components can be stored at room temperature (15-25℃) 並且至少穩定 18 在這些條件下幾個月.
評論
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