This product is suitable for rapid extraction of DNA from plant and fungal samples. The kit is based on silica gel column purification technology. The whole extraction process only takes 60-90 分分鐘. DNA can be used directly for 聚合酶鍊式反應, 定量PCR, southern Blot, test of virus DNA and so on.
細節
規格
| 特徵 | 規格 |
| 主要功能 | Isolation total DNA from 50mg simple plant using 96 well bind plate |
| 應用領域 | 聚合酶鍊式反應, SSR, AFLP, RAPD and southern blot, ETC. |
| 純化方法 | 96 孔板 |
| 淨化技術 | 二氧化矽技術 |
| 工藝方法 | 手動的 (離心或真空) |
| 樣品類型 | Economic plant samples |
| 樣品量 | Fresh / frozen plant samples: ≤50mg Dried plant / seed samples: ≤15mg |
| 洗脫體積 | ≥75μl |
| 每次運行時間 | 30-50 分分鐘 |
| 每根柱的載液量 | 800微升 |
| 柱結合率 | 100微克 |
原則
Plantmaterial is first mechanically disrupted and then lysed by addition of lysis buffer and incubation. 核糖核酸酶A in the lysis buffer digests the RNA in the sample. After lysis, proteins and polysaccharides are salt-precipitated. Binding buffer and ethanol are added to the cleared lysate to promote binding of the DNA to the HiPure membrane. The sample is then applied to a column and then centrifuged. DNA binds to the membrane, while contaminants such as proteins and polysaccharides are efficiently removed by 2 wash steps. Pure DNA is eluted in a small volume of low-salt buffer or water.
優點
- High quality DNA – meet a variety of downstream applications, 包括PCR, 定量PCR, 酵素消化, blot hybridization, ETC.
- 高通量 – 96 samples can be processed simultaneously by 96 孔板
- High permeability – using composite membrane technology, no hole plugging
套件內容
| 內容 | D316702 | D316703 |
| 淨化時間 | 4 X 96 準備工作 | 20 X 96 準備工作 |
| 核糖核酸酶溶液 | 5 毫升 | 22 毫升 |
| 洗脫緩衝液 | 120 毫升 | 500 毫升 |
| Buffer SPL | 200 毫升 | 2 X 500 毫升 |
| Buffer PS | 100 毫升 | 400 毫升 |
| 緩衝器GW1 | 220 毫升 | 5 X 220 毫升 |
| 緩衝器GW2 | 100 毫升 | 3 X 100 毫升 |
| 1.6ml Collection Plate | 4 | 20 |
| HiPure gDNA Plate | 4 | 20 |
| 2.2ml Collection Plate(DW Plate) | 8 | 40 |
| 0.8ml Collection Plate(DW Plate) | 4 | 20 |
| Sealing Film | 20 | 100 |
儲存和穩定性
RNase solution should be stored at 2-8°C upon arrival. 然而, 短期儲存 (取決於 24 週) 在室溫下 (15-25℃) 不影響其性能. 其餘套件組件可在室溫下乾燥保存 (15-25℃) 並且至少穩定 18 在這些條件下幾個月.
Purchase Guide
| 姓名 | CAT NO | Fresh / frozen tissue amount | Dried tissue amount | Column type | 洗脫體積 | 屈服 (肌肉) |
| HiPure Plant DNA Mini Kit | D3187 | 150毫克 | 40毫克 | 1.5ml column | 50 – 100微升 | 3-70微克 |
| HiPure HP Plant DNA Maxi Kit | D3163 | 5G | 1G | 50ml column | 0.7 – 3毫升 | 75-1250微克 |
| HiPure SF Plant DNA Kit | D3164 | 100毫克 | 20毫克 | 1.5ml column | 50 – 100微升 | 3-50微克 |
| HiPure SF Plant DNA 96 成套工具 | D3167 | 50毫克 | 15毫克 | 96 孔板 | 75 – 150微升 | 3-30微克 |
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