HiPure SF Plant DNA Kit

This product provides a fast and easy way to purify DNA from plant and fungal tissue. 取決於 100 mg of tissue can be processed. Easy-to-use Plant procedures provide pure total DNA (genomic,mitochondrial, and chloroplast) for reliable 聚合酶鍊式反應 and southern blotting in less than 1 小時. Purification requires no phenol or chloroform extraction oralcohol precipitation and involves minimal handling.

細節

規格

原則

Plantmaterial is first mechanically disrupted and then lysed by addition of lysis buffer and incubation. 核糖核酸酶A in the lysis buffer digests the RNA in the sample. After lysis, proteins and polysaccharides are salt-precipitated.Binding buffer and ethanol are added to the cleared lysate to promote binding of the DNA to the HiPure membrane. The sample is then applied to a column and then centrifuged. DNA binds to the membrane, while contaminants such as proteins and polysaccharides are efficiently removed by 2 wash steps. Pure DNA is eluted in a small volume of low-salt buffer or water.

優點

• 安全的 – require no phenol or chloroform extraction
• 快速地 – 可以提取多個樣本 30 minutes by silica technology
• 高純度 – high quality DNA, completely remove inhibitors
• 高產 – silica technology can achieve the highest yield

套件內容

儲存和穩定性

RNase A should be stored at 2-8°C upon arrival. 然而, 短期儲存 (取決於 24 週) 在室溫下 (15-25℃) 不影響其性能. 其餘套件組件可在室溫下乾燥保存 (15-25℃) 並且至少穩定 18 在這些條件下幾個月.

實驗數據

Purchase Guide