介紹
HiPure Soil RNA Kit is suitable for extracting high-purity microbial total RNA from soil samples. The kit adopts silica gel column purification technology and original humic acid adsorbent technology. It is suitable for extracting high-yield and high-purity total RNA from various soil samples, such as forest soil, grassland soil, mining soil, sediment and so on. The obtained RNA can be directly used in RT-聚合酶鍊式反應, Northern blot, poly-A + purification, 核酸保護與體外翻譯.
細節
規格
| 特徵 | 規格 |
| 主要功能 | Isolation total RNA from 500 mg soil sample |
| 應用領域 | 逆轉錄聚合酶連鎖反應, Northern blot, poly-A + purification, 核酸保護與體外翻譯 |
| 純化方法 | 小型的 旋轉柱 |
| 淨化技術 | 二氧化矽技術 |
| 工藝方法 | 手動的 (離心或真空) |
| 樣品類型 | Forest soil, grassland soil, mining area soil, sediment and other samples |
| 樣品量 | 500 毫克 |
| 洗脫體積 | ≥30μl |
| 每次運行時間 | ≤60分鐘 |
| 每根柱的載液量 | 800微升 |
| 柱結合率 | 100微克 |
原則
The hipure silica gel column is based on a glass fiber filter membrane with a high binding force. Under the condition of a high concentration of ionizing agent (例如 胍 hydrochloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bonds and electrostatic, while protein and other impurities are not adsorbed and removed. 清洗吸附有核酸的濾膜，去除殘留的蛋白質和鹽分. 最後, 吸附在濾膜上的核酸可以用低鹽緩衝液洗掉 (例如緩衝液TE) 或水. 所得的核酸純度高，可直接用於各種下游實驗.
優點
- 高品質 – high purity total RNA can be directly used in various sensitive downstream applications
- 快速地 – extraction of several samples can be completed in 60 minutes by the column purification method
- 安全的 – no phenol-chloroform extraction required
- 敏感的 – RNA can be purified at the level of PG
套件內容
| 內容 | R418302 | R418303 |
| 淨化時間 | 50 準備工作 | 250 準備工作 |
| HiPure RNA Micro Columns | 50 | 250 |
| 2毫升收集管 | 100 | 500 |
| gDNA Filter Column | 50 | 250 |
| 2ml Beads Tubes | 50 | 250 |
| Buffer SOL | 30 毫升 | 150 毫升 |
| 緩衝 安全資料表 | 4 毫升 | 15 毫升 |
| Buffer PHC | 30 毫升 | 150 毫升 |
| Buffer GDP | 40 毫升 | 150 毫升 |
| 緩衝器RW1 | 50 毫升 | 200 毫升 |
| Buffer RW2 * | 20 毫升 | 2 X 50 毫升 |
| 無核糖核酸酶水 | 10 毫升 | 30 毫升 |
儲存和穩定性
Buffer PHC should be stored at 2–8°C upon arrival. 然而, 短期儲存 (取決於 12 週) 在室溫下 (15–25°C) 不影響他們的表現. 其餘套件組件可在室溫下乾燥保存 (15–25°C) 並且至少穩定 18 在這些條件下幾個月. The entire kit can be stored at 2–8°C, but in this case, buffers should be redissolved before use. 使用時確保所有緩衝液均處於室溫.
實驗數據
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