- Isolation of High-Quality Genomic DNA: The product enables rapid and reliable isolation of high-quality genomic DNA from various soil and stool samples.
- Sample Processing Capacity: Capable of processing up to 100mg of stool samples and 500 mg of soil samples within 60 分分鐘.
- Innovative System Design: Combines the reversible nucleic acid binding properties of HiPure matrix with the speed and versatility of 旋轉柱 科技.
- Elimination of 聚合酶鍊式反應 Inhibitors: Effectively eliminates PCR-inhibiting compounds such as humic acid from soil samples during the purification process.
- Suitability for Various Applications: The purified DNA is suitable for PCR, 限制性消化, 和下一代定序.
- Reduction in Organic Extractions: No organic extractions are required, thus reducing plastic waste and hands-on time.
- Increased Efficiency: Allows for the processing of multiple samples in parallel, enhancing overall workflow efficiency.
規格
| 特徵 | 規格 |
| 主要功能 | Isolation DNA from 200-500mg soil, 50-100mg stool, or 100-500mg other environmental samples using 96 plate |
| 應用領域 | 聚合酶鍊式反應, southern blot and enzyme digestion, ETC. |
| 純化方法 | 96 孔板 |
| 淨化技術 | 二氧化矽技術 |
| 工藝方法 | 手動的 (離心或真空) |
| 樣品類型 | Soil, stool, other environmental samples |
| 樣品量 | Soil: 200-500毫克 Stool: 50-100毫克 Other environmental samples: 100-500毫克 |
| 洗脫體積 | |
| 每次運行時間 | |
| 每根柱的載液量 | |
| 柱結合率 |
原則
- Sample Homogenization and Lysis: Soil or stool samples are homogenized and treated in a specially formulated buffer containing detergent. This process effectively lyses bacteria, 酵母菌, and fungal samples.
- Removal of Contaminants: Our proprietary Absorber Solution is utilized to remove humic acid, 蛋白質, 多醣, and other contaminants from the lysed sample.
- DNA Binding and Column Application: After adjusting binding conditions, the treated sample is applied to a DNA Mini Column, facilitating the binding of DNA to the column matrix.
- Rapid Wash Steps: Two rapid wash steps are employed to remove trace contaminants, ensuring the purity of the extracted DNA.
- Elution of Purified DNA: Pure DNA is eluted from the column in a low ionic strength buffer, providing high-quality, purified DNA ready for downstream applications.
- Direct Use in Downstream Applications: The purified DNA can be directly used in downstream applications without the need for further purification steps, streamlining laboratory workflows and saving time.
優點
- 快速地 – 可以提取多個樣本 40 分分鐘 (after digestion)
- 高純度 – the purified DNA can be directly used in various downstream applications
- Good repeatability – silica technology can obtain ideal results every time
- 高回收率 – DNA can be recovered at the level of PG
套件內容
| 內容 | D314401 | D314402 | D314403 |
| 淨化時間 | 1 X 96 準備工作 | 4 X 96 準備工作 | 20 X 96 準備工作 |
| HiPure DNA Plate | 1 | 4 | 20 |
| 96 Well Plate (2.2毫升) | 1 | 4 | 20 |
| 1.6ml Collection Plate | 1 | 4 | 20 |
| 0.8ml Collection Plate | 1 | 4 | 20 |
| 2ml Bead Tubes | 100 | 400 | 2000 |
| Buffer SOL | 100 毫升 | 360 毫升 | 2 X 900 毫升 |
| 緩衝 安全資料表 | 10 毫升 | 36 毫升 | 180 毫升 |
| DX 試劑 | 1 毫升 | 1.8 毫升 | 9 毫升 |
| Buffer PS | 20 毫升 | 80 毫升 | 400 毫升 |
| Absorber Solution | 20 毫升 | 80 毫升 | 400 毫升 |
| Buffer GDP | 150 毫升 | 500 毫升 | 3 X 900 毫升 |
| Buffer GW2* | 50 毫升 | 100 毫升 | 4 X 200 毫升 |
| Buffer AE | 30 毫升 | 120 毫升 | 500 毫升 |
儲存和穩定性
- Absorber Solution Storage: 抵達時, the Absorber Solution should be stored at 2-8°C. 然而, it can also be stored at room temperature (15-25℃) for short-term periods (取決於 24 週) without affecting its performance.
- 其餘套件組件: The remaining components of the kit can be stored dry at room temperature (15-25℃). They remain stable for at least 18 在這些條件下幾個月, ensuring their efficacy and performance over an extended period.
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