HiPure Stool DNA Kit

Stool Sample in Molecular Biology Research:

1. Utility and Applications:
   • Stool samples are increasingly utilized in animal molecular genetics, population ecology, behavioral ecology, and intestinal disease diagnosis.
   • They contain gut microbial DNA, food residue sample DNA, and alimentary tract exfoliated cell DNA.
2. Challenges:
   • Low content of exfoliated cells in the digestive tract and degradation of genetic material pose primary challenges.
   • Presence of inhibitors such as polysaccharides, bile acids, bile salts, bile pigments, digestive juices, and mucus can affect 聚合酶鍊式反應-based molecular analysis.

Importance of DNA Extraction:

Critical Role:

• • Selecting appropriate extraction methods is crucial for obtaining high-quality DNA and ensuring successful downstream detection of stool DNA.

HiPure Stool DNA Kit:

1. Solution Provided:
   • The HiPure Stool DNA Kit from Magen Company offers a promising solution.
   • Features good universality, high cost-effectiveness, high yield, and purification.
2. Unique Features:
   • Utilizes a unique solution system and inhibitory factor adsorbent to efficiently remove various impurities in stool samples.
   • The purified DNA can be directly used for PCR, 定量PCR, 和其他應用.
3. Efficiency and Ease of Use:
   • Enables rapid and reliable isolation of high-quality genomic DNA from various stool samples.
   • Capable of processing up to 100 mg of stool samples within 60 分分鐘.
   • Combines reversible nucleic acid binding properties with 旋轉柱 科技, eliminating PCR inhibiting compounds.
   • Suitable for PCR, 限制性消化, and next-generation sequencing applications.
4. Sustainability and Scalability:
   • Eliminates the need for organic extractions, reducing plastic waste and hands-on time.
   • Allows multiple samples to be processed in parallel, enhancing efficiency.

細節

規格

原則

Stool DNA Extraction Process:

1. Homogenization and Lysis:
   • Stool sample is homogenized and treated in a specially formulated buffer containing detergent.
   • This step effectively lyses bacteria, 酵母菌, and fungal samples present in the stool.
2. Contaminant Removal:
   • Our proprietary Absorber Solution is used to remove humic acid, 蛋白質, 多醣, and other contaminants from the lysed sample.
3. Binding and Washing:
   • Adjusted binding conditions facilitate the attachment of DNA to a DNA Mini Column.
   • Two rapid wash steps are performed to remove trace contaminants, ensuring purity of the DNA.
4. Elution of Purified DNA:
   • Pure DNA is eluted from the column in a low ionic strength buffer.
   • The resulting purified DNA is ready for direct use in downstream applications without the need for further purification.

優點

• 高純度 – unique adsorbent can completely remove inhibitory factors
• 高濃度 – maximum extraction of total DNA from stool samples
• 高回收率 – DNA can be recovered at the level of PG
• Good repeatability – silica technology can obtain ideal results every time

套件內容

儲存和穩定性

Storage Instructions:

1. Proteinase K and Buffer PCI:
   • 抵達時, 儲存於2-8°C.
   • 短期儲存 (取決於 12 週) 在室溫下 (15-25℃) 不影響他們的表現.
2. 其餘套件組件:
   • 室溫保存 (15-25℃).
   • 至少穩定 18 在這些條件下幾個月.
3. Entire Kit:
   • Can be stored at 2–8°C.
   • Buffers should be redissolved before use if stored at this temperature.
   • Ensure all buffers are at room temperature when used.