產品概述
- Offers fast and easy methods for purification of total DNA.
- Ensures reliable 聚合酶鍊式反應 和Southern印跡.
- Capable of purifying various types of DNA, including genomic, viral, and mitochondrial.
- Suitable for purification from tissue and cultured cells.
規格
| 特徵 | 規格 |
| 主要功能 | Isolation of total DNA from <25 mg tissue, culture cells, FTA card |
| 應用領域 | 聚合酶鍊式反應, Southern Bolt virus detection, ETC. |
| 純化方法 | 小型的 旋轉柱 |
| 淨化技術 | 二氧化矽技術 |
| 工藝方法 | 手動的 (離心或真空) |
| 樣品類型 | Animal tissue or cultured cells |
| 樣品量 | 動物組織 : <25毫克, 培養細胞 : <5 X 106 |
| 洗脫體積 | ≥30μl |
| 每次運行時間 | 30 – 60 分分鐘 |
| 每根柱的載液量 | 800微升 |
| 柱結合率 | 100微克 |
原則
- Utilizes silica column purification method.
- 用裂解物和蛋白酶裂解和消化樣品, releasing DNA into the lysate.
- Lysate is transferred to an adsorption column.
- 核酸吸附在膜上, 而蛋白質不被吸附並透過過濾去除.
- Proteins and other impurities are washed away.
- Nucleic acid is finally eluted with low-salt buffer (10毫米三羥甲基氨基甲烷, 酸鹼度 9.0, 0.5乙二胺四乙酸).
優點
- Good repeatability – suitable for extracting high-yield DNA from different types of tissue samples
- 高純度 – can be used for downstream applications such as multiplex and quantitative PCR
- Fas t- simplified process, extracting several samples in 20 分分鐘 (after digestion)
- Safety – no phenol or chloroform extraction, no alcohol precipitation
套件內容
| 內容 | D312102 | D312103 |
| 淨化時間 | 50 準備工作 | 250 準備工作 |
| 緩衝區ATL | 15 毫升 | 65 毫升 |
| Buffer DL | 15 毫升 | 65 毫升 |
| 緩衝器GW1 | 22 毫升 | 110 毫升 |
| 緩衝器GW2 | 12 毫升 | 50 毫升 |
| 核糖核酸酶A | 10 毫克 | 50 毫克 |
| 蛋白酶K | 24 毫克 | 120 毫克 |
| 蛋白酶溶解緩衝液 | 5 毫升 | 15 毫升 |
| Buffer AE | 15 毫升 | 60 毫升 |
| HiPure gDNA Mini Columns | 50 | 2 X 125 |
| 2 毫升收集管 | 100 | 5 X 100 |
儲存和穩定性
- RNase A and Proteinase K should be stored at 2-8°C upon arrival.
- Short-term storage at room temperature (15-25℃) 最多為 24 weeks does not affect their performance.
- Remaining kit components can be stored dry at room temperature (15-25℃) 並且至少穩定 18 在這些條件下幾個月.
- 或者, the entire kit can be stored at 2-8°C, but buffers should be redissolved before use.
- Ensure all buffers are at room temperature when used.
評論
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