產品概述
- Offers fast and easy purification methods for total DNA.
- Ensures reliable 聚合酶鍊式反應 and Southern Blotting results.
- Suitable for purification of various types of DNA: genomic, viral, mitochondrial.
- Can purify DNA from tissue and cultured cells.
規格
| 特徵 | 規格 |
| 主要功能 | Isolation of total DNA from 2ml blood and 200mg tissue using Midi column |
| 應用領域 | 聚合酶鍊式反應, 南方螺栓和病毒檢測, ETC |
| 純化方法 | Midi 旋轉柱 |
| 淨化技術 | 二氧化矽技術 |
| 工藝方法 | 手動的 (離心或真空) |
| 樣品類型 | 組織, 細胞, 血, 唾液, 拭子, 血點, semen and other clinical samples |
| 樣品量 | 0.2-2 毫升 |
| 洗脫體積 | ≥300μl |
| 每次運行時間 | ≤80 minutes |
| 每根柱的載液量 | 4毫升 |
| 柱結合率 | 1毫克 |
Principles
- Utilizes silica column purification method.
- 用裂解物和蛋白酶裂解和消化樣品, releasing DNA into the lysate.
- Lysate is transferred to an adsorption column.
- 核酸吸附在膜上, while protein is not adsorbed and is removed through filtration.
- Proteins and impurities are washed away.
- Nucleic acid is finally eluted with low-salt buffer (10毫米三羥甲基氨基甲烷, 酸鹼度 9.0, 0.5乙二胺四乙酸).
優點
- High-quality DNA – meets a variety of downstream applications, 包括PCR, 定量PCR, 酵素消化, 雜交, ETC.
- 快速地 – without separation of leukocytes, 有機萃取, 或乙醇沉澱
- Simple – all nucleic acids can be obtained by direct digestion
- Wide applicability – handle a variety of liquid samples
套件內容
| 內容 | D311302 | D311303 |
| 淨化時間 | 20 | 100 |
| HiPure gDNA Midi Columns | 20 | 100 |
| 15毫升收集管 | 40 | 200 |
| 緩衝區ATL | 50 毫升 | 250 毫升 |
| 緩衝液AL | 50 毫升 | 250 毫升 |
| Buffer GW1* | 22 毫升 | 110 毫升 |
| Buffer GW2* | 12 毫升 | 50 毫升 |
| 核糖核酸酶A | 20 毫克 | 90 毫克 |
| 蛋白酶K | 100 毫克 | 440 毫克 |
| 蛋白酶溶解緩衝液 | 10 毫升 | 30 毫升 |
| Buffer AE | 20 毫升 | 120 毫升 |
儲存和穩定性
- Proteinase K and RNase A should be stored at 2-8°C upon arrival.
- Short-term storage at room temperature (15-25℃) 最多為 12 weeks does not affect their performance.
- 其餘試劑盒成分可在室溫下保存 (15-25℃) and remain stable for at least 18 在這些條件下幾個月.
實驗數據
Purchase Guide
| 姓名 | CAT NO | 樣品量 | Leukocyte protocol* | Colum type | Elutio volume | Average yield | 每次運行時間 |
| HiPure 血液 DNA 迷你試劑盒 | D3111 | 10-200微升 | 1毫升 | 2ml column | ≥20μl | 5-9μg/200μl | ≤30 minutes |
| HiPure 組織&血液 DNA 中型套件 | D3113 | 0.2-2毫升 | 10毫升 | 1.5ml column | ≥300μl | 20-40μg/1m | ≤80 minutes |
| HiPure 組織&血液 DNA 馬克西套件 | D3115 | 3 -10毫升 | 10毫升 | 15ml column | ≥700μl | 20-40μg/1m | ≤90 minutes |
| HiPure 組織&血液DNA 96 成套工具 | D3117 | 1-200微升 | 1毫升 | 96 孔板 | 3-8μg/200μl |
評論
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