The HiPure Total RNA Kit provides fast purification of high-quality RNA from cells, 組織, and yeast using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or isopropanol precipitation. RNA purified using the HiPure Total RNA Purification System can be used for applications such as RT-聚合酶鍊式反應, 北方印跡, poly A+ RNA (信使RNA) purification, nuclease protection, and in vitro translation.
細節
規格
| 特徵 | 規格 |
| 主要功能 | 分離總RNA (not include miRNA) from animal tissues, cells and simple plant tissues using one column |
| 應用領域 | 逆轉錄聚合酶連鎖反應, 實時螢光定量PCR, 北方雜交, 二代定序 |
| 純化方法 | 小型的 旋轉柱 |
| 淨化技術 | 二氧化矽技術 |
| 工藝方法 | 手動的 (離心或真空) |
| 樣品類型 | Animal soft tissue, 培養細胞, 淋巴球, simple plant tissue |
| 樣品量 | 細胞: ≤1 x 107 動物組織: 1-20 毫克 植物葉子: 50-150 毫克 Yeast cells: 5 X 106 |
| 屈服 | 2-100微克 |
| 洗脫體積 | ≥50μl |
| 每次運行時間 | ≤15 minutes（1-24 samples） |
| 每根柱的載液量 | 800微升 |
| 柱結合率 | 100微克 |
原則
HiPure RNA technology simplifies total RNA isolation. Samples are first lysed and then homogenized. Ethanol is added to the lysate to provide ideal binding conditions. The lysate is then loaded onto the HiPure silica membrane and RNA binds to the silica membrane, and all contaminants are efficiently washed away. For certain RNA applications that are sensitive to very small amounts of DNA, the residual amounts of DNA remaining can be removed using a convenient on-column DNase treatment. Pure, concentrated RNA is eluted in water.
優點
- Efficient removal of DNA – unique genomic DNA removal column without DNase treatment
- 高品質 – high purity total RNA can be directly used in various sensitive downstream applications
- 快速地 – 可以提取多個樣本 20 分鐘柱法
- 安全的 – 無需苯酚氯仿萃取
- 敏感的 – RNA can be recovered at the level of PG
套件內容
| 內容 | R401102 | R401103 |
| 淨化時間 | 50 準備工作 | 250 準備工作 |
| HiPure RNA 迷你柱 | 50 | 250 |
| 2毫升收集管 | 50 | 250 |
| RTL Lysis Buffer | 50 毫升 | 200 毫升 |
| RNA Binding Buffer | 15 毫升 | 75 毫升 |
| 緩衝器RW1 | 50 毫升 | 200 毫升 |
| 緩衝器 RW2* | 20 毫升 | 2 X 50 毫升 |
| 無核糖核酸酶水 | 10 毫升 | 30 毫升 |
儲存和穩定性
The Kit can be stored dry at room temperature (15-25℃) 並且至少穩定 18 在這些條件下幾個月.
評論
還沒有評論.