This kit provides fast purification of high-quality RNA from cells, 組織, and yeast using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or isopropanol precipitation. RNA purified using the HiPure Total RNA Purification System can be used for applications such as RT-聚合酶鍊式反應, 北方印跡, poly A+ RNA (信使RNA) purification, nuclease protection, and in vitro translation.
細節
規格
| 特徵 | 規格 |
| 主要功能 | 分離總RNA (not include miRNA) from 20mg tissue, 150mg plant, 5 X 106 cell using two columns (gDNA removed column) |
| 應用領域 | 逆轉錄聚合酶連鎖反應, 實時螢光定量PCR, 北方雜交, 二代定序 |
| 純化方法 | 小型的 旋轉柱 |
| 淨化技術 | 二氧化矽技術, DNA filtration technology |
| 工藝方法 | 手動的 (離心或真空) |
| 樣品類型 | Animal soft tissue, 培養細胞, 淋巴球, simple plant tissue |
| 樣品量 | 細胞:≤1 x 107 動物組織樣本: 1-20 毫克 Plant tissue: 50-150 毫克 |
| 屈服 | 2-100微克 |
| 洗脫體積 | ≥50μl |
| 每次運行時間 | ≤25 minutes（1-24 samples） |
| 每根柱的載液量 | 800微升 |
| 柱結合率 | 100微克 |
原則
The Kit isolates total RNA from up to 107 cells or 20 mg tissue. A short workflow enables RNA isolation with genomic DNA removal in less than 25 分分鐘. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column, ethanol is added to the flow-through, and the sample is applied to a RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 30µl water using the Kit.
優點
- Efficient removal of DNA – unique genomic DNA removal column without DNase treatment
- 高品質 – high purity total RNA can be directly used in various sensitive downstream applications
- 快速地 – 可以提取多個樣本 25 分鐘柱法
- 安全的 – 無需苯酚氯仿萃取
- 敏感的 – RNA can be recovered at the level of PG
套件內容
| 內容 | R411102 | D411103 |
| 淨化時間 | 50 準備工作 | 250 準備工作 |
| HiPure DNA Mini Columns | 50 | 250 |
| HiPure RNA 迷你柱 | 50 | 250 |
| 2毫升收集管 | 100 | 2 X 250 |
| Buffer RLC | 50 毫升 | 200 毫升 |
| 緩衝器RW1 | 50 毫升 | 200 毫升 |
| 緩衝器 RW2* | 12 毫升 | 2 X 50 毫升 |
| 無核糖核酸酶水 | 10 毫升 | 30 毫升 |
儲存和穩定性
HiPureKit can be stored dry at room temperature (15-25℃) and are stable for at least18 months under these conditions. During shipment, crystals or precipitationmay form in the Buffer RLC. Dissolve by warming buffer to 37°C.
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