This product offers a convenient and rapid method to purify total DNA from a wide range of samples, making it ideal for various downstream analyses:
- Fast and Efficient: The protocol is streamlined for quick DNA extraction with minimal hands-on time.
- High-Quality DNA: The system isolates total DNA (genomic, viral, mitochondrial) with high purity, suitable for reliable 聚合酶鍊式反應, 定量PCR (定量PCR), 南方印跡, and viral DNA detection.
- 廣泛的樣品相容性: The method effectively purifies DNA from a variety of sources, 包括:
- 組織
- 細胞
- 血
- 唾液
- 拭子
- 血斑
- 精液
- Other clinical samples
- Downstream Applications: The purified DNA can be used directly in various research techniques, 包括:
- 聚合酶鍊式反應 (聚合酶鍊式反應)
- 定量PCR (定量PCR)
- Southern印跡法
- Viral DNA Detection
- 其他基於 DNA 的分析
This solution simplifies DNA extraction while maintaining high quality, making it a valuable tool for researchers working in various fields of molecular biology, 法醫, and diagnostics.
規格
| 特徵 | 規格 |
| 主要功能 | Isolation of total DNA from tissue/blood/body fluid/swab/dry blood spots |
| 應用領域 | 聚合酶鍊式反應, 定量PCR, 南方螺栓和病毒檢測, ETC |
| 純化方法 | 小型的 旋轉柱 |
| 淨化技術 | 二氧化矽技術 |
| 工藝方法 | 手動的 (離心或真空) |
| 樣品類型 | 組織, 細胞, 血, 唾液, 拭子, 血點, 精液, 和其他臨床樣本 |
| 樣品量 | 實體組織: 1-10毫克; 抗凝血: 200微升 |
| 洗脫體積 | ≥20μl |
| 每次運行時間 | 30 – 60 分分鐘 |
| 每根柱的載液量 | 800微升 |
| 柱結合率 | 100微克 |
原則
- Purification Method: Utilizes silica column purification for efficient nucleic acid extraction.
- Sample Lysis and Digestion: Samples are lysed and digested using lysate and protease, facilitating the release of DNA into the lysate.
- Nucleic Acid Adsorption: The lysed sample is transferred to an adsorption column, where nucleic acids selectively adsorb onto the membrane.
- Protein Removal: Proteins, which do not adsorb to the membrane, are removed through filtration, ensuring purity of the extracted nucleic acid.
- Washing Step: Proteins and other impurities are washed away from the membrane to enhance the purity of the extracted nucleic acid.
- 洗脫: Nucleic acid is finally eluted from the membrane using a low-salt buffer (10毫米三羥甲基氨基甲烷, 酸鹼度 9.0, 0.5乙二胺四乙酸), resulting in efficient recovery of high-quality nucleic acid.
優點
- High-quality DNA – meets a variety of downstream applications, 包括PCR, 定量PCR, 酵素消化, 雜交, ETC.
- 快速地 – without separation of leukocytes, 有機萃取, 或乙醇沉澱
- Simple – all nucleic acids can be obtained by direct digestion
- Wide applicability- handle a variety of liquid samples
套件內容
| 內容 | D301802 | D301803 |
| 淨化時間 | 50 | 250 |
| HiPure DNA 迷你柱 I | 50 | 250 |
| 2毫升收集管 | 100 | 500 |
| 緩衝區ATL | 30 毫升 | 150 毫升 |
| 緩衝液AL | 30 毫升 | 150 毫升 |
| 緩衝器GW1 | 22 毫升 | 88 毫升 |
| 緩衝器GW2 | 12 毫升 | 50 毫升 |
| 蛋白酶K | 24 毫克 | 120 毫克 |
| 蛋白酶溶解緩衝液 | 1.8 毫升 | 10 毫升 |
| Buffer AE | 15 毫升 | 60 毫升 |
儲存和穩定性
- 蛋白酶 K 儲存: 抵達時, store Proteinase K at 2-8°C. 短期儲存 (取決於 12 週) 在室溫下 (15-25℃) 在不影響性能的情況下可以接受.
- Remaining Kit Components Storage: 其餘試劑盒成分可在室溫下保存 (15-25℃) and remain stable for at least 18 在這些條件下幾個月.
- Whole Kit Storage: The entire kit can also be stored at 2-8°C. 然而, in this case, buffers should be redissolved before use. Ensure all buffers are at room temperature when used for optimal performance.
實驗數據
