MagPure A3 Features:
- Technology Base:
- Utilizes Magen’s solid-phase paramagnetic bead technology for the purification of 聚合酶鍊式反應 amplicons.
- High-Throughput Compatibility:
- Designed for high-throughput applications, facilitating efficient processing of multiple samples.
AmPure Technology:
- Selective Binding:
- Features an optimized buffer that selectively binds PCR amplicons of 100bp and larger to paramagnetic beads.
- Contaminant Removal:
- Efficiently removes excess primers, 核苷酸, 鹽類, and enzymes through a simple washing procedure.
- Purity of PCR Product:
- Delivers PCR products that are essentially free of contaminants, enhancing downstream application reliability.
細節
規格
特徵 | 規格 |
主要功能 | Selectively recover DNA from PCR products and enzymatic reaction solution (Replace Beckmen or agencourt AmPure) |
應用領域 | 定序, gene chip and qPCR, ETC. |
淨化技術 | 磁珠技術 |
工藝方法 | 手動的 (離心或真空) |
樣品類型 | PCR products, 酵素反應溶液 |
樣品量 | 合適的 |
洗脫體積 | ≥10μl |
運行時間 | ≤50分鐘 |
原則
Product Purification Method:
- High Binding Magnetic Particle Method:
- Utilizes high binding magnetic particles for purification.
- PCR Amplicon Binding:
- PCR amplicons are mixed with MagPure A3.
- DNA fragments of 100bp and larger bind to magnetic beads.
- Contaminant Removal:
- Excess primers, 核苷酸, 鹽類, and enzymes are removed via a simple washing procedure.
- DNA Elution:
- Purified DNA is eluted using Elution Buffer or Water.
優點
- Wide application – DNA產品, enzyme digestion system, PCR reaction
- 高回收率 – suitable for 100bp-20kb fragments
- 高純度 – effectively remove dNTP, primer, primer dimer, salt ion and other impurities
- Strong applicability – manual operation or automatic workstation
套件內容
內容 | BXP-5 | BXP-50 | BXP-500 |
MagPure A3 | 5 毫升 | 50 毫升 | 500 毫升 |
儲存和穩定性
Storage and Handling Instructions:
- 儲存條件:
- Store MagPure A3 at 2-8°C upon arrival.
- Stable for up to 18 在這些條件下幾個月.
- Short-term Storage:
- 短期儲存 (取決於 12 週) 在室溫下 (15-25℃) does not affect performance.
- 試劑製備:
- Shake the reagent well before use to ensure homogeneity.
- The reagent should appear homogenous and consistent.
不要凍結.
評論
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