MagPure A4 XP Features:
- Technology Base:
- Employs Magen’s solid-phase paramagnetic bead technology designed for the purification of 聚合酶鍊式反應 amplicons.
- High-Throughput Capability:
- Suitable for high-throughput settings, allowing for efficient simultaneous processing of multiple samples.
AmPure Technology:
- Selective Binding:
- Uses an optimized buffer that selectively binds PCR amplicons 100bp and larger to paramagnetic beads.
- Contaminant Removal:
- Simple washing procedure efficiently removes excess primers, 核苷酸, 鹽類, and enzymes.
- Purity of PCR Product:
- Results in PCR products that are virtually free from contaminants, suitable for precise downstream applications.
細節
規格
特徵 | 規格 |
主要功能 | Selectively recover DNA from PCR products and enzymatic reaction solution (Replace Beckmen or agencourt AmPure XP) |
應用領域 | NGS, DNA library |
淨化技術 | 磁珠技術 |
工藝方法 | 手動的 (離心或真空) |
樣品類型 | DNA產品, 限制性內切酶系統, or other enzymatic reaction solutions |
樣品量 | 合適的 |
恢復 | 80% |
運行時間 | ≤50分鐘 |
原則
Purification Method:
- High Binding Magnetic Particle Method:
- Utilizes high binding magnetic particles for purification.
- PCR Amplicon Binding:
- PCR amplicons are mixed with MagPure A3.
- DNA fragments of 100bp and larger bind to magnetic beads.
- Contaminant Removal:
- Excess primers, 核苷酸, 鹽類, and enzymes are removed through a simple washing procedure.
- DNA Elution:
- Purified DNA is eluted using Elution Buffer or Water.
優點
- High recovery — up to 80%
- High throughput — using magnetic beads purification technology
套件內容
內容 | BP-5 | BP-50 | BP-500 |
MagPure A4 XP | 5 毫升 | 50 毫升 | 500 毫升 |
儲存和穩定性
Storage and Handling Instructions:
- 儲存條件:
- Store MagPure A4 XP at 2-8°C upon arrival.
- Stable for up to 18 在這些條件下幾個月.
- Short-term Storage:
- 短期儲存 (取決於 4 週) 在室溫下 (15-25℃) does not affect performance.
- 試劑製備:
- Shake the reagent well before use to ensure homogeneity.
- The reagent should appear homogeneous and consistent in color.
不要凍結.
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