This product provides fast and easy methods for the purification of total DNA for reliable 聚合酶鍊式反應 and southern blotting. Total DNA (例如, genomic, plasmid) can be purified from bacterial cultures.
規格
| 特徵 | 規格 |
| 主要功能 | Isolation total DNA from bacterial culture |
| 應用領域 | 聚合酶鍊式反應, southern blot and enzyme digestion, ETC. |
| 淨化技術 | 磁珠技術 |
| 工藝方法 | 手動或自動 |
| 樣品類型 | Gram negative or positive bacteria |
| 樣品量 | Bacterial culture: 0.5-1.5毫升 |
| 洗脫體積 | ≥60μl |
| 每次運行時間 | ≤60分鐘 |
原則
- This product utilizes high-binding magnetic particles for purification.
- Samples are lysed and digested using a lysate and Lysozyme.
- DNA is released into the lysate during this process.
- Magnetic particles and binding solution are added, allowing DNA to be adsorbed onto the surface of the particles while impurities such as proteins are not adsorbed.
- The adsorbed particles undergo washing with a washing solution to remove proteins and impurities.
- Ethanol is used for further washing to remove salts.
- 最後, DNA is eluted using Elution Buffer.
優點
- 快速地: Multiple samples can be extracted within 60 分分鐘 (after digestion), making the process time-efficient.
- 高純度: Unique adsorbents ensure the complete removal of inhibitory factors, resulting in high-purity DNA extraction.
- 高回收率: DNA can be recovered at the level of picograms (PG), indicating a high level of efficiency in the extraction process.
套件內容
| 內容 | D513101 | D513102 | D513103 |
| 淨化時間 | 48 準備工作 | 96 準備工作 | 480 準備工作 |
| MagPure 顆粒 | 1.2 毫升 | 2.5 毫升 | 11 毫升 |
| 溶菌酶 | 60 毫克 | 120 毫克 | 600 毫克 |
| 蛋白酶K | 24 毫克 | 50 毫克 | 220 毫克 |
| 蛋白酶溶解緩衝液 | 3 毫升 | 6 毫升 | 30 毫升 |
| 緩衝 安全資料表 | 1.5 毫升 | 3 毫升 | 15 毫升 |
| Buffer MLA | 30 毫升 | 60 毫升 | 300 毫升 |
| Buffer GW1* | 13 毫升 | 44 毫升 | 110 毫升 |
| Buffer TE | 30 毫升 | 40 毫升 | 200 毫升 |
儲存和穩定性
- MagPure 顆粒, 溶菌酶, and Proteinase K should be stored at 2-8°C upon arrival.
- 短期儲存 (取決於 12 週) 在室溫下 (15-25℃) 不影響他們的表現.
- 其餘套件組件可在室溫下乾燥保存 (15-25℃) and remain stable for at least 18 在這些條件下幾個月.
評論
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