MagPure Circulating DNA Maxi Kit

介紹

This kit empowers researchers with a streamlined solution for isolating high-quality circulating cell-free DNA (遊離DNA) from various biofluids:

• Sample Types: 電漿, 血清 (1-6毫升)
• 目標: cfDNA molecules
• Downstream Applications:
  • 聚合酶鍊式反應 (polymerase chain reaction)
  • 實時定量PCR
  • Biochip analysis
  • 新一代定序 (NGS)

規格

原則

This product utilizes innovative magnetic bead technology for efficient DNA extraction:

1. 裂解和消化: The sample undergoes treatment with a special solution (lysate) and an enzyme (protease) to break open cells and degrade proteins, releasing DNA into the lysate.
2. Binding to Magnetic Beads: Magnetic particles coated for high DNA affinity are added to the lysate. DNA molecules selectively bind to the surface of these beads, while impurities like proteins remain unbound.
3. 洗滌和淨化: A series of washes with different solutions remove unbound proteins and other cellular debris.
4. 洗脫: A special elution buffer disrupts the interaction between the DNA and the magnetic beads, allowing the purified DNA to be collected in a separate tube.

優點

• Economy – less than 50% of the price of Qiagen and other imported products
• Automatic – without labor
• 高純度 – OD 260 / 280: 1.7-1.9, OD 260 / 230:1.5-2.0

套件內容

儲存和穩定性

理想的存儲:

• Store Proteinase K, 載體RNA, and MagPure Particles F in the refrigerator (2-8℃) 抵達後發揮最大功效.

可接受的短期儲存:

• These components can be kept at room temperature (15-25℃) 最多為 12 幾週而不影響他們的表現.

儲存剩餘組件:

• All other kit components can be stored dry at room temperature (15-25℃).
• 他們至少會保持穩定 18 在這些條件下幾個月.

重要提示:

• 如果將整個套件儲存在 2-8°C, 使用前記得重新溶解緩衝液.
• 使用前請務必確保所有緩衝液達到室溫以獲得最佳結果.

實驗數據