Fast and Easy Total DNA純化
- Objective: Reliable 聚合酶鍊式反應 and Southern Blotting
- 特徵:
- Swift purification methods
- Easy-to-follow protocols
- 好處:
- Saves time and effort
- Ensures reliable PCR and Southern blotting results
- Applicability:
- Purifies various types of total DNA:
- Genomic
- Viral
- Mitochondrial
- Suitable for diverse sample types:
- 全血
- 電漿
- 血清
- Buffy coat
- Bone marrow
- Other body fluids
- Lymphocytes
- 培養細胞
- Purifies various types of total DNA:
規格
| 特徵 | 規格 |
| 主要功能 | Isolation of total DNA from 200μl whole blood |
| 應用領域 | 聚合酶鍊式反應, 南方螺栓和病毒檢測, ETC |
| 淨化技術 | 磁珠技術 |
| 工藝方法 | 手動或自動 |
| 樣品類型 | 全血, concentrated blood, 白膜層, and other samples |
| 樣品量 | 200微升 |
| 洗脫體積 | ≥70μl |
| 每次運行時間 | ≤40分鐘 |
原則
- Sample lysis and digestion facilitated by lysate and Protease.
- DNA 釋放到裂解液中.
- DNA adsorption onto magnetic particles’ surface, excluding impurities like proteins.
程式:
- Sample lysis and digestion: Lysate and Protease act on the sample.
- DNA release: DNA 釋放到裂解液中.
- DNA adsorption: Magnetic particles and binding solution are added, facilitating DNA adsorption.
- Impurity removal: Proteins and impurities are removed without DNA adsorption.
- 洗滌:
- 第一的, wash with washing solution to remove proteins and impurities.
- Ethanol wash to eliminate salts.
- 洗脫: DNA is eluted using an Elution Buffer.
優點:
- High specificity: Selective DNA binding, excluding impurities.
- 效率: Rapid purification process.
- Minimal sample loss: Enhanced DNA recovery.
應用領域:
- Ideal for PCR and Southern blotting experiments requiring purified DNA.
- Suitable for various sample types and DNA sources.
優點
- High Binding Force: Magnetic beads with exceptional binding capacity efficiently capture DNA molecules even from samples with abundant DNA, such as whole blood, 白膜層, and concentrated blood.
- Rapid Processing: The use of polydisperse magnetic beads ensures a fast magnetic response, leading to significantly reduced extraction times.
- High-Purity DNA: The optimized protocol delivers highly purified DNA, eliminating the need for further purification steps. This high-quality DNA is directly usable for various downstream applications:
- Second-generation sequencing (NGS)
- PCR-based detection methods
- Gene bank construction
- And other DNA analyses requiring high purity
套件內容
| 內容 | D631001 | D631002 | D631003 |
| 淨化時間 | 48 | 96 | 480 |
| MagPure 顆粒 | 1.7 毫升 | 3.5 毫升 | 17 毫升 |
| 蛋白酶K | 24 毫克 | 50 毫克 | 220 毫克 |
| 蛋白酶溶解緩衝液 | 1.8 毫升 | 3 毫升 | 15 毫升 |
| Buffer MLA | 30 毫升 | 60 毫升 | 270 毫升 |
| Buffer DW1 | 60 毫升 | 120 毫升 | 550 毫升 |
| Buffer CW | 30 毫升 | 60 毫升 | 270 毫升 |
| Buffer BW3 | 30 毫升 | 60 毫升 | 270 毫升 |
| 洗脫緩衝液 | 15 毫升 | 30 毫升 | 100 毫升 |
儲存和穩定性
蛋白酶K, MagPureParticles should be stored at 2-8°C upon arrival. 然而, 短期儲存(取決於 24 週) 在室溫下 (15-25℃) 不影響他們的表現. 其餘試劑盒成分可在室溫下保存 (15-25℃)並且至少穩定 18 在這些條件下幾個月.
