產品概述
- Suitable for rapid DNA extraction from FFPE 樣品.
- Utilizes two combination methods: High-salt Bind and Alcohol mediated adsorption.
- High-salt Bind removes pigments or polysaccharides from complex FFPE samples, enhancing nucleic acid purity and preventing blockages in aligent 2100.
- Alcohol-mediated adsorption improves nucleic acid yield in high-yield samples.
規格
| 特徵 | 規格 |
| 主要功能 | Isolation of high pure total DNA from FFPE using high bind beads |
| 應用領域 | 聚合酶鍊式反應 and viral DNA detection, ETC. |
| 淨化技術 | 磁珠技術 |
| 工藝方法 | 手動或自動 |
| 樣品類型 | Paraffin-embedded tissue samples |
| 樣品量 | 1-6 slices of 10-20μm |
| 洗脫體積 | ≥30μl |
| 每次運行時間 | ≤60分鐘 |
原則
優點
- 高產 – 最優過程, recovery up to 90%
- Economy – less than 50% of the price of Qiagen and other imported products
- 高純度 – OD 260/280: 1.7-1.9, OD 260/230: 1.5-2.0
套件內容
| 內容 | D632301D | D632302D |
| 淨化時間 | 48 準備工作 | 96 準備工作 |
| MagPure 顆粒 N | 1.1 毫升 | 2.5 毫升 |
| 核糖核酸酶A | 10 毫克 | 20 毫克 |
| 蛋白酶K | 24 毫克 | 48 毫克 |
| 蛋白酶溶解緩衝液 | 3 毫升 | 6 毫升 |
| Buffer DPS | 60 毫升 | 100 毫升 |
| 緩衝區ATL | 15 毫升 | 30 毫升 |
| Buffer BST1 | 30 毫升 | 60 毫升 |
| Buffer BW1 | 13 毫升 | 44 毫升 |
| 洗脫緩衝液 | 15 毫升 | 30 毫升 |
儲存和穩定性
- 蛋白酶K, 核糖核酸酶A, and MagPure Particles N should be stored at 2-8°C initially.
- Short-term storage at room temperature (15-25℃) 最多為 12 weeks doesn’t affect their performance.
- Remaining kit components can be stored at room temperature and remain stable for at least 18 在這些條件下幾個月.
評論
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