介紹
The Kit is specially designed for simultaneous purification of genomic DNA and total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. Purified DNA/RNA is suitable for use in applications such as real-time 聚合酶鍊式反應 and Pyrosequencing.
細節
規格
| 特徵 | 規格 |
| Main FunctionsC | Co-isolation total RNA and DNA from FFPE tissue |
| 應用領域 | 逆轉錄聚合酶連鎖反應, cDNA synthesis, PCR and second-generation sequencing, ETC. |
| 純化方法 | 多分散磁珠 |
| 淨化技術 | 磁珠技術 |
| 工藝方法 | 手動或自動 |
| 自適應儀器 | Nucleic acid extractor and pipetting workstation |
| 樣品類型 | FFPE slice, FFPE puncture sample, embedded tissue |
| 樣品量 | No more than six 10µm sections of 150 毫米2 surface area or three 20µm sections of 150 毫米2 surface area. |
原則
FFPE samples are incubated in an optimized lysis buffer, which results in the release of RNA and precipitation of DNA. 離心後, the RNA-containing supernatant and DNA-containing pellet are then processed separately to purify RNA and DNA. 加入磁性顆粒和結合溶液後, DNA/RNA會被吸附在磁性顆粒的表面, 蛋白質等雜質不會被吸附而被去除. 吸附的顆粒用洗滌液洗滌，除去蛋白質和雜質, 用乙醇洗滌以除去鹽, and finally DNA/RNA was eluted by RNase Free Water.
優點
- Post digestion sorting – higher DNA and RNA yields
- Economy – the price is much lower than imported reagents
套件內容
| 內容 | IVD3026 |
| 淨化時間 | 200 準備工作 |
| 結合粒子 | 9.0 毫升 |
| 蛋白酶K | 180 毫克 |
| 蛋白酶溶解緩衝液 | 10 毫升 |
| Buffer DPS | 150 毫升 |
| Buffer FRL | 40 毫升 |
| 緩衝區ATL | 40 毫升 |
| 緩衝液AL | 80 毫升 |
| Buffer BXW1* | 110 毫升 |
| 無核糖核酸酶水 | 30 毫升 |
儲存和穩定性
Proteinase K and MagBind Particles should be stored at 2-8°C upon arrival. 然而, 短期儲存 (取決於 24 週) 在室溫下 (15-25℃) 不影響他們的表現. 其餘試劑盒成分可在室溫下保存 (15-25℃) 並且至少穩定 18 在這些條件下幾個月.
評論
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