介紹
The kit offers the unique feature to isolate total RNA including small RNA and DNA from serum and plasma without the need to resort to the cumbersome phenol/chloroform extraction or a time consuming proteinase digest. RNA purified using the kit is ready for applications such as RT-聚合酶鍊式反應, 北方印跡, poly A+ RNA (信使RNA) purification, nuclease protection, and in vitro translation.
細節
規格
| 特徵 | 規格 |
| 主要功能 | Isolation miRNA from 0.3-0.5ml serum or plasma using magnetic particles |
| 應用領域 | 逆轉錄聚合酶連鎖反應, cDNA synthesis, 二代定序 |
| 純化方法 | 多分散磁珠 |
| 淨化技術 | 磁珠技術 |
| 工藝方法 | 手動或自動 |
| 自適應儀器 | 核酸萃取儀, 移液工作站 |
| 樣品類型 | 血清, 電漿, acellular samples |
| 樣品量 | 300微升 |
原則
本產品以高結合磁性顆粒的純化方法. The sample material is denatured in Lysis Buffer. The protein is then precipitated by Protein Precipitation Solution and pelleted by centrifugation. 加入磁性顆粒和結合溶液後, RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption.The adsorbed particles were washed with washing solution to remove proteins and impurities, 用乙醇洗滌以除去鹽, and finally RNA was eluted by Elution Buffer.
優點
- 安全的 – no phenol chloroform extraction and no use of Trizol reagent
- 快速地 – 可以提取多個樣本 60 分鐘柱法
套件內容
| 內容 | R662801 | R662802 | R662803 |
| 淨化時間 | 48 準備工作 | 96 準備工作 | 5 X 96 準備工作 |
| MagPure 顆粒 N | 1.7 毫升 | 3.5 毫升 | 17 毫升 |
| Buffer CFL | 6 毫升 | 12 毫升 | 60 毫升 |
| Buffer CPL | 1.8 毫升 | 3.5 毫升 | 20 毫升 |
| Buffer MGW1* | 30 毫升 | 60 毫升 | 250 毫升 |
| 緩衝器 MW2* | 12 毫升 | 25 毫升 | 100 毫升 |
| 無核糖核酸酶水 | 10 毫升 | 20 毫升 | 60 毫升 |
儲存和穩定性
MagPure Particle N should be stored at 2–8°C upon arrival. 然而, 短期儲存 (取決於 8 週) 在室溫下 (15–25°C) 不影響他們的表現. 其餘試劑盒成分可在室溫下保存 (15–25°C) 並且至少穩定 18 在這些條件下幾個月.
評論
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