介紹
This product is suitable for rapid extraction of DNA from tissue, 細胞, 血, 唾液, 拭子, 血點, 精液, 和其他臨床樣本. DNA can be used directly for 聚合酶鍊式反應, 定量PCR, Southern Blot, test of virus DNA, and so on.
細節
規格
| 特徵 | 規格 |
| 主要功能 | Isolation of total DNA from blood, 白膜層, 組織, and other samples |
| 應用領域 | Second-generation sequencing, 聚合酶鍊式反應, real-time PCR, ETC. |
| 純化方法 | 多分散磁珠 |
| 淨化技術 | 磁珠技術 |
| 工藝方法 | 手動或自動 |
| 樣品類型 | 抗凝血, concentrated blood, 白膜層, lymphocytes and cultured cells |
| 樣品量 | 全血 :< 200微升; 唾液 / swab:< 400微升; 組織 :< 20毫克 |
| 屈服 | 0.1 – 50微克 |
| 洗脫體積 | |
| 每次運行時間 |
原則
This product is based on the purification method of high-binding magnetic particles. The sample is lysed and digested under the action of lysate and protease. DNA 釋放到裂解液中. 加入磁性顆粒和結合溶液後, DNA will be adsorbed on the surface of magnetic particles, 蛋白質等雜質不會被吸附而被去除. The adsorbed particles were washed with a washing solution to remove proteins and impurities, 用乙醇洗滌以除去鹽, and finally, DNA was eluted with an Elution Buffer.
優點
- 高純度 – OD 260 / 280: 1.7- 1.9, OD 260 / 230: 1.5 – 2.0
- Economy – less than 50% of the price of Qiagen and other imported products
- 高產 – 最優過程, ensuring the recovery of up to 90%
- Strong processing ability – samples including animal blood, 培養細胞, 動物組織, ETC.
套件內容
| 內容 | IVD3102 |
| 淨化時間 | 200 |
| MagPure 顆粒 | 5 毫升 |
| 蛋白酶K | 100 毫克 |
| 蛋白酶溶解緩衝液 | 10 毫升 |
| 核糖核酸酶A | 40 毫克 |
| 緩衝區ATL | 60 毫升 |
| 緩衝液AL | 60 毫升 |
| Buffer BD* | 20 毫升 |
| Buffer BW1* | 110 毫升 |
| 洗脫緩衝液 | 30 毫升 |
儲存和穩定性
蛋白酶K, 核糖核酸酶A, MagPure Particles should be stored at 2-8°C upon arrival. 然而, 短期儲存 (取決於 24 週) 在室溫下 (15-25℃) 不影響他們的表現. 其餘試劑盒成分可在室溫下保存 (15-25℃) 並且至少穩定 18 在這些條件下幾個月.
實驗數據
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