指示:
- 樣本採集:
- Collect 0.1-1ml blood sample.
- 樣品製備:
- 添加 2 到 3 times the volume of 1x red blood cell solution to the blood sample.
- Mix thoroughly by inverting.
- 離心機在 12,000 轉速為 1 分分鐘.
- Carefully aspirate the supernatant. The precipitate should ideally be white or light red.
- 添加 400 μl reagent buffer I and 10 μl epoxy resin K (10 毫克/毫升) to the precipitate.
- Vortex and mix for 15 秒, then let it sit for 2 分分鐘.
- Special Case: Low-level Organisms:
- If processing blood from low-level organisms (例如, 家禽, birds) containing cell nuclei:
- Add 1x red blood cell solution and directly add 200 μl of reagent buffer I (total volume not exceeding 500 微升).
- Let it sit for 2 分分鐘.
- Digestion:
- 添加 10 μl epoxy resin K (10 毫克/毫升) to the mixture.
- Invert and mix, then digest at 65°C for 10 分分鐘.
- During this period, 反轉並混合 6-7 times to ensure complete digestion.
- 轉移至試劑板:
- Add the solution from the previous step to the 96-well reagent plate.
- 按照自動提取方案中概述的具體步驟進行進一步處理.
