- 樣品處理:
- 收集 0.1-1 ml blood sample.
- 樣品製備:
- For blood samples:
- 添加 2-3 times the volume of 1x erythrocyte lysate.
- Fully invert and mix well.
- 離心機在 12,000 轉速為 1 分分鐘.
- Carefully remove the supernatant. The precipitate should be white or light red.
- 添加 400 μl reagent buffer I and 10 μl proteinase K (10 毫克/毫升) to the precipitate.
- Vortex and mix for 15 秒, then incubate at room temperature for 2 分分鐘.
- For blood samples:
- Special Consideration for Low-Level Biological Blood:
- If processing poultry, 鳥, or other low-level biological blood with nuclei-containing red blood cells and low sample volume:
- Skip adding 1x erythrocyte lysate.
- Directly add 200 μl of reagent buffer I (total volume not exceeding 500 微升).
- Incubate at room temperature for 2 分分鐘.
- If processing poultry, 鳥, or other low-level biological blood with nuclei-containing red blood cells and low sample volume:
- Digestion:
- 添加 10 μl proteinase K (10 毫克/毫升) to the mixture.
- Mix thoroughly by inversion.
- Digest at 65°C for 10 分分鐘, inverting and mixing 6-7 times during digestion until complete.
- 轉移至試劑板:
- Add the lysate from the previous step to the 96-well reagent plate.
- 按照自動提取方案中概述的具體步驟進行進一步處理.
Pre-processing of blood sample genomic DNA extraction 96 樣品
$225.00
運費美元 45 - 超過美元免費 300DTE是一家專門從事分子檢測線上銷售的中國電子商務平台, 酵素連結免疫吸附試驗, 及相關產品.
- 製造商: 中國領導品牌
- 船運: 直接從工廠加急聯邦快遞發貨
- 符合退貨或換貨條件 30 天
- 支付方式: 安全的 PayPal 或信用卡.
描述
附加資訊
| 重量 | 0.7 公斤 |
|---|---|
| 品牌 |