產品概述
聚合酶鍊式反應 amplification of DNA fragments, DNA labeling, primer extension, sequence determination, blunt end addition of A, TA vector cloning
| 產品資訊 | |
|---|---|
| 分子量 | 94 肯德基 |
| Polymerase Activity | 5′-3′ DNA聚合酶, 5′-3′ exonuclease (no 3′-5′ exonuclease) |
| PCR Buffer | 10 X PCR Buffer for direct loading |
| Experimental Efficiency | The PCR reaction can be directly detected by electrophoresis |
| Extension Speed | 1-2 kb/min |
| Terminal Base | Product has A at the 3′ 結尾, facilitating TA vector cloning |
| Reaction System (20微升) | Addition Amount | 評論 |
|---|---|---|
| Pure DNA Polymerase | 0.8微升 | Adjust for amplification needs |
| 10x PCR Buffer | 2微升 | |
| 脫氧核糖核酸 (2.5毫米) | 2微升 | |
| PrimerF | 0.1-1微升 | 10-50pmol used |
| PrimerR | 0.1-1微升 | 10-50pmol used |
| Template | <1微克 | |
| ddH2O | Up to 20μl |
| Cycle Program | 溫度 (℃) | 時間 | 評論 |
|---|---|---|---|
| Initial Denaturation | 94 | 3分分鐘 | |
| 變性 | 94 | 30s (30-40 週期) | |
| 退火 | 45-60 | 30s | |
| 擴大 | 72 | 30s-2min | Extension time varies with fragment |
| 最終延期 | 72 | 10分分鐘 |
程式
Can be directly detected without adding a Loading Buffer
Result Detection
PCR後, 拿 5 μl of product for agarose gel electrophoresis
評論
還沒有評論.