規格
| 屬性 | 細節 |
|---|---|
| 貯存 | 2-8℃ |
| 單元 | Box |
| 有效期限 | 6 月 |
| 英文名 | Pepsase Activity Assay Kit |
| Detection Method | 分光光度計/酶標儀 |
| 規格 | 100T/48S |
產品構成
| 試劑名稱 | 規格 | Storage Condition |
|---|---|---|
| 萃取液 | 液體 50 mL×1瓶 | 4℃保存 |
| 試劑一 | Powder×1 bottle | 4℃保存 |
| 試劑二 | 液體 15 mL×1瓶 | 4℃保存 |
| 試劑三 | Powder×1 bottle | 4℃保存 |
Instructions for preparing the solutions:
- Solution One: 添加 10 mL of Reagent Two before use and dissolve completely.
- Solution Three: 添加 10 mL of distilled water before use and dissolve completely.
產品描述:
- Pepsin is secreted by the chief cells of the gastric mucosa and hydrolyzes proteins in food into small peptides. It is commonly used for the identification of hypochlorhydria, and reduced secretion of pepsin may also occur in conditions such as chronic gastritis, chronic gastric dilation, and chronic duodenitis.
- Pepsin catalyzes the hydrolysis of hemoglobin, and the hydrolysis product tyrosine exhibits a characteristic absorption peak at 275nm. Enzyme activity is calculated by measuring the changes in absorbance.
Instruments and supplies required:
- UV spectrophotometer/enzyme marker
- Benchtop centrifuge
- Water bath
- Adjustable pipette
- Micro quartz cuvette/96-well UV plate
- Mortar and pestle
- Ice
- 蒸餾水
筆記: It is recommended to perform a pilot experiment with 2-3 samples showing significant expected differences before conducting the actual experiment. If the absorbance values of the samples are not within the measurement range, dilution or increasing the sample volume for detection is recommended.
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