Solarbio 血清鐵離子含量測定試劑盒

血清鐵離子含量測定試劑盒

筆記: 測試前取兩個或三個不同的樣本進行預測.

操作設備: 分光光度計

Cat Number: BC1730

尺寸: 50T/48S

成分:

試劑一: 粉末×2, 4℃保存. 添加 10 使用前毫升蒸餾水.

試劑Ⅱ: 粉末×2, 4℃保存. 添加 313 μL glacial acetic acid and 10 使用前毫升蒸餾水. Standard Solution: 液體 3 毫升×1, 1000 μmol/L Fe3+ standard solution, 4℃保存. Add distilled water dilute 8 times to form a standard solution of 125 μmol/L before use.

產品描述:

Serum iron is the iron bound with transferrin in blood, which is often used to distinguish non-iron deficiency anemia and iron-deficiency anemia.

Fe3+ is reduced by sodium sulfite to Fe2+, which reacts with 2,2-dipyridine-bipyridine, have an absorption peak at 520 奈米. According measure absorbance at 520 nm can reflect serum iron concentration.

需要但未提供的試劑和設備.

分光光度計, 離心機, glacial acetic acid, adjusted transferpettor, 1 毫升玻璃比色皿, chloroform and distilled water.

程式:

1. 預熱分光光度計 30 分分鐘, 調整波長至 520 奈米, 用蒸餾器調零
2. Dilute standard solution to 125 μmol/L with distilled
3. 加入如下清單的試劑:

計算

Serum iron (微摩爾/公升)=[Cs×(AT-AB)÷(As-AB)]= 125×(AT-AB)÷(As-AB)

Cs: Fe3+ standard solution, 125 微摩爾/公升.

筆記:

1. There is less iron in the serum, so the vessels (EP tubes) should be noted to avoid iron contamination.
2. Reagent I and Reagent II are unstable. It needs to be prepared when the solution will be used, and the newly prepared reagent can only be used on the same day.
3. When At- Ac＞5, please dilute the Serum to appropriate concentration with distilled water.

技術規格：

最低檢測極限：3.212 微摩爾/毫升

線性範圍：3.9-250 微摩爾/毫升

相關產品：

BC2860/BC2865 Serum Total Iron Binding Capacity(TIBC) 檢測試劑盒

BC0720/BC0725 Blood Calcium Content Assay Kit