血清鐵離子含量測定試劑盒
筆記: 測試前取兩個或三個不同的樣本進行預測.
操作設備: 分光光度計
Cat Number: BC1730
尺寸: 50T/48S
成分:
試劑一: Powder×2, 4℃保存. 添加 10 使用前毫升蒸餾水.
試劑Ⅱ: Powder×2, 4℃保存. 添加 313 μL glacial acetic acid and 10 使用前毫升蒸餾水. Standard Solution: 液體 3 毫升×1, 1000 μmol/L Fe3+ standard solution, 4℃保存. Add distilled water dilute 8 times to form a standard solution of 125 μmol/L before use.
產品描述:
Serum iron is the iron bound with transferrin in blood, which is often used to distinguish non-iron deficiency anemia and iron-deficiency anemia.
Fe3+ is reduced by sodium sulfite to Fe2+, which reacts with 2,2-dipyridine-bipyridine, have an absorption peak at 520 奈米. According measure absorbance at 520 nm can reflect serum iron concentration.
需要但未提供的試劑和設備.
分光光度計, 離心機, glacial acetic acid, adjusted transferpettor, 1 毫升玻璃比色皿, chloroform and distilled water.
程式:
- 預熱分光光度計 30 分分鐘, 調整波長至 520 奈米, 用蒸餾器調零
- Dilute standard solution to 125 μmol/L with distilled
- 加入如下清單的試劑:
| 試劑名稱 (微升) | 空白管 (AB) | 試管 (在) | 標準管 (作為) |
| 蒸餾水 | 400 | ||
| 標準溶液 (125 微摩爾/公升) | 400 | ||
| 血清(電漿) | 400 | ||
| 試劑一 | 400 | 400 | 400 |
| 試劑二 | 400 | 400 | 400 |
| 充分混合, incubate in boiling water bath for 5 分分鐘, cooling liquid. 添加 200 μL chloroform (required but not provided). 充分混合, room temperature, 10000 rpm centrifuge for 10 分分鐘. 拿 700 μL supernatant to 1 毫升玻璃比色皿. Measure absorbance at 520 奈米. Recorded as AB, 在, 作為. | |||
計算
Serum iron (微摩爾/公升)=[Cs×(AT-AB)÷(As-AB)]= 125×(AT-AB)÷(As-AB)
Cs: Fe3+ standard solution, 125 微摩爾/公升.
筆記:
- There is less iron in the serum, so the vessels (EP tubes) should be noted to avoid iron contamination.
- Reagent I and Reagent II are unstable. It needs to be prepared when the solution will be used, and the newly prepared reagent can only be used on the same day.
- When At- Ac＞5, please dilute the Serum to appropriate concentration with distilled water.
技術規格：
最低檢測極限：3.212 微摩爾/毫升
線性範圍：3.9-250 微摩爾/毫升
相關產品：
BC2860/BC2865 Serum Total Iron Binding Capacity(TIBC) 檢測試劑盒
BC0720/BC0725 Blood Calcium Content Assay Kit
評論
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