介紹
- The products undergo rigorous screening and quality control to serve as the primary raw material for nucleic acid extraction.
- Buffer solutions are capable of resisting the impact of strong acids and bases, thereby maintaining a stable pH within the system. It’s crucial to uphold a pH range of 4.0 到 11.0 during nucleic acid extraction.
- Nucleic acids are prone to hydrolysis in acidic conditions but remain stable in neutral or weakly alkaline environments. Tris, or trimethylolaminomethane, functions as a pH buffer within the range of 7.0 到 9.0. Its role is to preserve the stability of nucleic acids post-sample cleavage, preventing degradation and enhancing concentration and purity.
- Tris-containing reagents cannot undergo treatment with DEPC, necessitating rigorous assurance of RNase absence during Tris preparation. 通常, DEPC-treated water is employed for preparation to ensure RNase-free Tris of high purity, exceeding 99.99%.
- This product undergoes stringent testing for RNase and DNase activity, confirming its purity of over 99.99% and absence of DNase or RNase activity.
細節
規格
特徵 | 規格 | |
基本內容 | 推薦應用 | 核酸萃取 |
CAS 編號 | 1185-53-1 | |
公式 | C4H11NO3.HCL | |
分子量 | 157.60 | |
穩定 | 乾燥條件下無限制 | |
等級 | 分子生物學 | |
外貌 | 白色晶體 | |
純度 | ≥99% | |
運輸條件 | 室內溫度 | |
保存條件 | 室內溫度 | |
雜質參數 | Smell | Nothing |
Melting point ℃ | 145-155 | |
Heavymetal | <5 PPM | |
Losson drying | ≤0.09% | |
紫外線吸收值 | 吸光度值@ 230 (5%) | ≤0.01 |
吸光度值@ 260 (5%) | ≤0.01 | |
吸光度值@ 280 (5%) | ≤0.01 | |
吸光度值@ 320 (5%) | ≤0.01 | |
核酸萃取相關 | 脫氧核糖核酸酶測試 (0.5中號) | 沒有檢測到 |
核糖核酸酶測試 (0.5中號) | 沒有檢測到 | |
PH值 (2中號) | 4.0-5.0 |
訂購資訊
目錄號. | 產品名稱 | 包裹 |
C11224 | Tris-HCl, TRIS hydrochloride (分子生物學) | 1公斤 |
C11225 | 5公斤 |
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