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主要組成及內容:
| 成分 | ED2304001-01（24T） | ED2304001-02（50T） |
| Fluorescent 聚合酶鍊式反應 reaction solution | 500μL per tube × 1 管子 | 1200μL per tube × 1 管子 |
| 陽性對照 | 50μL per tube × 1 管子 | 200μL per tube × 1 管子 |
| 陰性對照 | 50μL per tube × 1 管子 | 200μL per tube × 1 管子 |
| 手動的 | 1 | 1 |
功能和目的:
用於檢測包括全血在內的各種樣本中的目標核酸, 血清, 淋巴結, 脾, 肌肉, 和別的.
劑量和測定:
1 使用說明
1.1 樣品加工
Process the samples according to relevant standards and store the processed samples for later use.
1.2 Experimental Procedure
1.2.1 試劑準備區
Remove the reagent kit, take out the required reagents for the experiment, thaw and mix thoroughly, then briefly centrifuge for 5 seconds to remove any liquid adhering to the tube walls;
Add 20μL of fluorescent PCR reaction solution into each PCR tube and transfer it to the sample preparation area.
1.2.2 樣品製備區
Add 5μL each of the negative control, the nucleic acid of the sample to be tested, and positive control. Briefly centrifuge for 5 seconds and transfer to the amplification area.
1.2.3 Amplification Area
Place each PCR tube in the corresponding positions of the instrument’s sample slots and record the placement sequence.
Set the instrument’s nucleic acid amplification parameters according to the table below and perform the PCR amplification.
| Reaction System | 25μL Reaction System | ||
| Signal Channel | FAM Channel for Collecting Fluorescent Signals | ||
| PCR Reaction Conditions | Stage | Conditions | 週期 |
| Pre-Denaturation | 95℃：3分分鐘 | 1 | |
| 聚合酶鍊式反應 | 95℃：15秒 | 40 | |
| 60℃：30秒 | |||
2 結果解讀
2.1 Establishment of Experimental Conditions:
2.1.1 Positive Control: CT值≤ 30, showing clear exponential growth, displaying a typical “S” curve.
2.1.2 Negative Control: Ct value > 38 or no Ct value, showing no clear exponential growth phase or plateau.
2.2 Criteria for Determination:
2.2.1 Positive: Sample detection result Ct value ≤ 35, displaying clear exponential growth, indicating the presence of the target DNA in the sample.
2.2.2 疑似: Sample detection result Ct value between 35 和 38; the sample should be retested. If the repeated experiment results in Ct values still between 35 和 38 with clear exponential growth, 它被認為是積極的; 否則, it’s negative.
2.2.3 消極的: Sample detection result Ct value > 38 or no Ct value, indicating the absence of the target DNA in the sample.
預防措施:
- Before the experiment, 仔細閱讀本試劑盒說明書，嚴格遵守操作流程操作.
- Store all reagents at the specified temperatures; detailed information is available on the reagent labels.
- Sample handling should occur in a biosafety cabinet. 實驗結束後, 所有使用的樣品和材料均經過高溫滅菌.
- To prevent cross-contamination, work in an environment as free from nucleases as possible, and segment the experimental process (試劑準備區, 樣品製備區, 放大面積, ETC。).
- When preparing the PCR reaction system, try to avoid bubble formation. Before amplification, check if reaction tubes are tightly sealed to prevent leakage and contamination of the instrument.
- This kit can be used with standard nucleic acid extraction purification methods for sample testing, maintaining constant judgment parameters such as Ct values.
- To ensure accurate experimental results, samples should be freshly collected and transported at 2-8°C; 用於長途運輸, use dry ice.
- Avoid repeated freezing and thawing of all reagents.
規格: 24 時間/盒子, 50 時間/盒子
Storage and Shelf Life: 儲存於-20°C, away from light. Shelf life is 12 月.
