什麼是 NEB Taq DNA 聚合酶?

內布泰DNA聚合酶

NEB Taq DNA Polymerase is a type of DNA polymerase enzyme produced by New England Biolabs (NEB) that is commonly used for DNA amplification using the Polymerase Chain Reaction (聚合酶鍊式反應). It is a recombinant form of Taq polymerase, which is derived from the bacterium Thermus aquaticus.

NEB Taq DNA Polymerase is highly thermostable and can withstand high temperatures, which makes it ideal for use in PCR, where high-temperature cycles are used to amplify DNA. It exhibits high processivity and fidelity, which means it can replicate DNA strands with high accuracy and efficiency. NEB Taq DNA polymerase is enhanced with hot start capabilities, which prevent non-specific amplification and primer-dimer formation during PCR, leading to more specific and sensitive results.

NEB Taq DNA Polymerase is provided as a master mix containing the Taq polymerase enzyme, dNTP, and other buffer components required for PCR. It is available in different formats, including Taq DNA Polymerase with ThermoPol Buffer, Taq DNA Polymerase with ThermoPol II Buffer, and Taq DNA Polymerase with GC Buffer, each designed to optimize amplification of different types of DNA templates.

NEB Taq DNA Polymerase is a reliable and widely-used tool for PCR amplification of DNA, and has contributed significantly to the advancements in molecular biology research.

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